Abelmoschus esculentus (L.) Moench polysaccharide, a promising protectant enhances Caco-2 cell adhesive and immune-modulatory competence of Bifidobacterium longum MYL16
碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 103 === Abelmoschus esculentus (L.) Moench is one of commonly used vegetable in Asia. It was established that the Abelmoschus esculentus (L.) Moench has an anti-tumor cancer, antioxidant, antibacterial, anti-allergy, blood sugar/cholesterol lowering, and other bioa...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2015
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| Online Access: | http://ndltd.ncl.edu.tw/handle/64404498489037425456 |
| Summary: | 碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 103 === Abelmoschus esculentus (L.) Moench is one of commonly used vegetable in Asia. It was established that the Abelmoschus esculentus (L.) Moench has an anti-tumor cancer, antioxidant, antibacterial, anti-allergy, blood sugar/cholesterol lowering, and other bioactivity. It is little is known that okra polysaccharides can be used as probiotics protective agent.
In this study, Abelmoschus esculentus (L.) Moench as a polysaccharide extracted source, to discuss polysaccharides extracted from Abelmoschus esculentus (L.) Moench can improve the survival rate of Bifidobacterium longum MYL16 under freeze-drying and enhance its ability to gastric acid and bile salts of resistance in artificial gastrointestinal tract.
The result shows that adding 20% Abelmoschus esculentus (L.) Moench polysaccharide mixed 10% or 20% skim milk can maintain Bifidobacterium longum MYL16 activity for eight weeks. In addition, add a polysaccharide of the test group survival bacteria higher than the group did not add polysaccharide in freeze-drying test. This study confirms by adding okra polysaccharides can improve survival rate of Bifidobacterium in freeze-drying. It can improve Bifidobacterium to resist gastric acid and bile salts and also improve adsorption of Caco-2 and immunomodulatory capacity. Abelmoschus esculentus (L.) Moench has tremendous development potential of probiotic protective agent.
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