| Summary: | 碩士 === 國立中興大學 === 材料科學與工程學系所 === 103 === Nanomaterials of special physical and chemical properties have been applied to biomedical fields such as anti-bacteria products, drug carriers and anti-cancer drugs, etc. However, as the possibility of contact with nanoparticles (NPs) increases, the cytotoxicity of NPs (especially Ag) to cells (such as red blood cells, RBCs) needs to be evaluated. It is highly demanded to clarify the cytotoxicity of Ag NPs to the morphology, mechanical properties and cytoskeleton of RBCs. Thus in this study, we observed the changes in the morphology and cytoskeleton of RBCs under the influences of Ag NPs, and analyzed the variations of cytoskeleton and mechanical behavior by using a micropippte aspiration. Experimental results indicated that the Ag-NP-affected RBCs became echinocyte-like rather than normal biconcave, and the crosslinking density and diameter of cytoskeleton increased. In the micropipette aspiration of live RBCs, the Ag-NP-affected RBCs (whole cell) showed a shorter projection length, a longer aspiration time, and a higher elastic modulus than normal RBCs did. Moreover, in the micropipette aspiration of actin stained RBCs, the fluorescence area of normal RBCs obviously decreased, whereas the area of the Ag-NP-affected RBCs was nearly the same, and the aggregated cytoskeleton hardly moved. In summary, under the influence of Ag NPs, RBCs hardly deformed (with a longer aspiration time and a higher elastic modulus) in consequence of cytoskeleton aggregation and thickening.
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