Comparative histological analysis on testicular atrophy and early recrudescence of White Leghorn Chicken and White Roma Goose

• Main Authors: Lu-Yu Lu, 呂鹿榆
• Other Authors: 鄭旭辰
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/05634762855195500674

碩士 === 國立中興大學 === 生命科學系所 === 103 === For animals with seasonal reproduction, their gonads are well developed and producing gametes during breeding season; the gonads undergo atrophy and apoptosis while entering the non-breeding season. This change is regulated by the hypothalamic–pituitary–gonadal (HPG) axis such that seasonal breeders experience annual cycles of reproductive quiescence and renaissance. White Roman gooses are short-day breeder, they have reproductive activity in winter and gonad regress in summer. Wild chicken are long-day breeder, their reproductive periods are in summer and in winter the gonad undergo regression . White Leghorn chicken are well domesticated breeder with relatively pure genetic background and individual differences are smaller than other avian species. Therefore, White Leghorn chicken is an ideal animal model for investigating the genetic and molecular mechanisms of cellular changes of gonad regression and recrudescence. However White Leghorn chicken has no seasonal reproduction and will require further comparative analysis seasonal breeders to ensure proper application. In our experiments we use forced-molting to change Leghorn rooster gonad state, and testes were collected in different time point during forced-molting；while goose testicular tissues are harvested according to their nature annual cycle of reproduction. The testis size dropped 35% in chicken and dropped 86.5% in goose and testicular structures were analyzed with IHC，we found both of their somatic cells and germ cells were decreased during testicular regression and apoptotic cells were increased in rooster testes. When testes revived, both of their somatic cell and germ cell were increased and total proliferating cell in testes also expand. According these results, we concluded that artificial induced gonadal change and naturally gonadal change have similarly process.