Establishment of multiplication protocol on medicinal plant Dysosma pleiantha (Hance) Woodson

• Main Authors: Kai-Siang Lin, 林凱翔
• Other Authors: Song-Iuan Liaw
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/26501395452776081646

碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 103 === The purpose of this test is therefore to examine the use of the tissue culture technology called micropropagation for reproducing the medicinal plant Dysosma and building its multiplication system, which involves methods and processes including preparation of sterile explants, blastemal induction, multiplication, rooting, hardening and transplanting. For the induction of wound healing tissues, placing the petioles of the plant in a culture medium that contained BA 0.2mg.L-1+2,4-D 0.5 mg.L-1 produced the best inductive effect, with an induction rate up to 88.2%. For blastemal induction, incubation using a growth regulator that contained BA 1.0 mg.L-1+TDZ 0.1 mg.L-1 achieved the highest induced blastemal fractionation rate, up to 33.2%. For blastemal multiplication, it was found that treating with BA/NAA in a concentration gradient of 10:1 (0.1-2.0 mg.L-1/0.01-0.2 mg.L-1) produced the largest number of blastemas, with an average of 8.5. In the rooting test, incubation using a culture medium that contained 1/2 MS+ IBA 0.5 mg.L-1 was able to induce rooting in the second week with an induction rate of about 20%, which increased to 100% in the fourth week. In the hardening and transplanting test, explants that had grown roots for at least six weeks were used and all of them, could be successfully transplanted and had a survival rate of 100%.