Investigation of the impact of environmental exposure to plasticizers on metabolic activation of estrogen and developmental function of newborn children in Taiwanese pregnant women

• Main Authors: Chia-Wei Liu, 劉家瑋
• Other Authors: Po-Hsiung Lin
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/25396401911729210707

碩士 === 國立中興大學 === 環境工程學系所 === 103 === Both 17β-estradiol-2,3-quinone (E2-2,3-Quinone) and 17β-estradiol-3,4-quinone (E2-3,4-Quinone) are reactive metabolites of estrogen that are thought to be responsible for the estrogen-induced genotoxicity. The aim of this study was to analyze estrogen quinone-derived adducts in hemoglobin (Hb) derived from pregnant women (n=270) and to investigate the associations of these adducts with levels of phthalate metabolites in urine and growth and development of newborns. Results confirmed that levels of estrogen quinone-derived adducts in pregnant women hemoglobin. E2-3,4-Q-2-Hb and E2-2,3-Q-4-Hb were detected (n=270) with median levels at 157.6 (range 108.3-410.8), 92.8 (range 62.7-228.0) pmol/g, respectively. Levels of E2-3,4-Q-2-Hb correlated significantly with those of E2-2,3-Q-4-Hb (correlation coefficient r=0.634, p<0.001). The urinary MnBP (Mono-n-benzyl phthalate) negatively correlated with those of E2-3,4-Q-2-S-Hb (r=-0.158, p=0.023) and E2-2,3-Q-4-S-Hb (r=-0.141, p<0.043) in pregnant women. Further investigation indicated that chest circumference and finger length of newborns positively correlated with levels of E2-3,4-Q-2-S-Hb (r=0.245 and 0.34, respectively, p<0.05). Overall, this evidence suggests that the background level of these adducts in human hemoglobin associates with environmental exposure to DBP and that maternal blood levels of estrogen quinones associate with children hormonal control and reproductive development. In conclusions, estrogen quinone-derived hemoglobin adducts may be applied to epidemiological studies as biomarkers of estrogen homeostasis