Treatment efficiency and microorganism community variation of an Anammox-MSBR nitrogen removal system under low nitrogen loading

• Main Authors: Chih-Chun Chang, 張智鈞
• Other Authors: Chun-Hsiung Hung
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/14037582491390972245

碩士 === 國立中興大學 === 環境工程學系所 === 103 === Petro-chemical and High-Tech industries grow rapidly in Taiwan. These industries generate significant amount of nitrogen-containing pollutants, such as ammonium while during the manufacturing process. These pollutants would cause seriously environmental pollution and danger human health without appropriate treatment. Although, many kinds of nitrogen removal treatments were discovered, biological nitrogen removal from wastewater is the friendliest and harmless techniques to environment. Among them, anaerobic ammonium oxidation (Anammox) process is a newly discovered microbial nitrogen removal treatment process. This autotrophic mediating anammox process not only could convert ammonium and nitrite directly into nitrogen gas, but also could consume carbon dioxide under anoxic environment. Furthermore, the operating cost is less than the traditional nitrification/denitrification wastewater treatment process. Therefore, research and application of anammox process have become increasingly popular in the field of environmental engineering. In this study, a lab-scale pilot Anammox-MSBR (membrane sequencing batch reactor) system was used to treat low nitrogen concentration (ammonium about 50 mg-N/L) wastewater from Science Park and the new effluent standard of ammonium (30 mg-N/L). During the operation time, water quality, microbial community structure and interaction microorganisms were detected for monitoring and discussing the variation of nitrogen loading and nitrogen removal efficiency. Results showed that removal efficiency of ammonium, nitrite and the total inorganic nitrogen are 99%, 90%, and 60%, respectively. On the other hand, Real-Time PCR analysis showed that the quantification of gene copies of anammox microorganisms maintain at 109 copies/µg DNA. Others nitrogen-related microorganisms like ammonium oxidation bacteria and denitrifying bacteria are 104 copies/µg DNA and 106 copies/µg DNA, respectively. Besides, this study also discovered that the nitrogen removal efficiency was decreased suddenly under high nitrate concentration. Despite of nitrate cause damage the nitrogen removal efficiency of anammox system. After adjusting the incubation to the optimal situation for anammox growth and metabolism, the anammox system had positive results on the system performance. At the same time, Anammox microorganisms had been changed from Candidatus Brocadia and Candidatus Kuenenia into a co-existing patterns of Candidatus Brocadia、Candidatus Kuenenia and Candidatus Anammoxoglobus propionicus while under the low nitrogen loading condition. The treatment of low nitrogen loading was achieved by using Anammox-MSBR system and the nitrogen removal rate of this system is about 0.128 kg-N/m3/day. Sum up, this experimental setup is successfully improved nitrogen loading to the same as the real wastewater and have full-scale application potentials.