Synthesis of calcium phosphate hybrids with PAMAM dendrimer by electrophoretic deposition and its application in drug release

• Main Authors: Yin-Hsuan Chen, 陳尹宣
• Other Authors: Hui-Ting Chen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/465ffw

碩士 === 高雄醫學大學 === 香粧品學系碩士班 === 103 === The study was development of organic/inorganic hybrid materials by mimetic biomineralization. In this work, polyamidoamine (PAMAM) dendrimers with various functional groups were used as organic templates to form PAMAM/calcium phosphate (CaP) hybrid materials through electrophoretic deposition. It was found that the PAMAM/CaP hybrids show globular shape determined by transmission electron microscope (TEM) and scanning electron microscope (SEM). As concentration of PAMAM was increased from 3x10-4 to 3x10-2 M, the particle sizes of composites were reduced from 3 μm to 0.3 μm. At a fixed concentration of organic template, the different types of terminal functional groups on PAMAM can also affect the PAMAM/CaP particle sizes. The order of particles size is PAMAM-mPEG/CaP > PAMAM-NH2/CaP > PAMAM-COOH/CaP. Moreover, the method was convenient to encapsulate simvastatin in PAMAM/CaP composite and its encapsulation ratio is 77%. This material was incubated for 7 days at pH 7.4 to release 4.9% of simvastatin, and the release ratio can be increased to 30.5% at pH 5.5, so this material is suitable to release drug under acidic condition. This study develops a method which can rapidly prepare organic/ inorganic composite and the encapsulate drug into the composite material, making it more potential for clinical uses.