Investigation of pH-sensitive liposomal formulation of Mitomycin C for drug delivery system

• Main Authors: Pei-Yu Hu, 胡培瑜
• Other Authors: Yi-Ping Fang
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/v3vq32

碩士 === 高雄醫學大學 === 藥學系碩士班 === 103 === Mitomycin C is a potent anticancer drug. But the severe side effect like cumulative myelosuppression and nephrotoxicity limit its therapeutic efficacy. Moreover, the clinical use of Mitomycin C is significantly hindered due to the rapid elimination from the body. The half-life of Mitomycin C is about 23~78 minutes. The purpose of this study was to formulate the pH-sensitive liposomes for cancer therapy. DOPE, CHEMS and DSPE-PEG2000-folate were used as materials to prepare pH-sensitive liposomes. The physicochemical characteristics of pH-sensitive liposomes : particle size 144.5±2.8 nm, polydispersity index 0.343±0.047, zeta potential -66.7±0.3 mV and entrapment efficiency 66.5±0.1%. In vitro drug release was modestly prolonged and presented good pH sensitivity. In cytotoxicity assays, the IC50 of pH-sensitive liposomes was 8.75 &;#61549;M. The half-life of pH-sensitive liposomes and Mitomycin C solution were 1.35±0.15 and 1.60±0.04 hours. Besides, the blood toxicity observations of rats would be revealed.