| Summary: | 碩士 === 高雄醫學大學 === 藥學系碩士班 === 103 === An efficient, simple, sensitive and rapid on-line sample concentration technique－field-amplified sample stacking-sweeping-micellar electrokinetic chromatography (FASS-sweeping-MEKC) was developed for simultaneous determination of para-cresol sulfate (PCS) and 3-indoxyl sulfate (3-INS) in human serum. PCS and 3-INS are the two important representatives of endogenous “protein-bound uremic toxins” and would accumulate in chronic renal failure patients with impaired kidney functions, leading to various adverse impacts and toxicities on human body. Therefore, detection of PCS and 3-INS are getting more and more attention in recent years at clinic. In this study, qualitative and quantitative analysis of PCS and 3-INS in human serum can be achieved under the optimized conditions. Separation was carried out in phosphate buffer (100 mM, pH 4.0) containing 150 mM SDS and 10% MeOH at 214 nm within 10 min. Detection limit (S/N=3) was found to be 100 ng/mL for PCS and 30 ng/mL for 3-INS. Calibration curves showed great linearity (r≧0.99) and small RSD and RE values (≦3.47%) in intra-day and inter-day assays demonstrated the good precision and accuracy of the CE method. Furthermore, the developed method has been successfully applied to analyze real samples from hemodialysis patients, aiming to be helpful for disease evaluation and become as an objective indicator for medication treatment in patients.
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