Metal Characterization of Alloys and Imaging Analysis of Tissues Using Laser Desorption Ionization Mass Spectrometry

• Main Authors: Christopher Stephen Shiea, 謝中浩
• Other Authors: Yeou-Lih Huang
• Format: Others
• Language: en_US
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/223y46

碩士 === 高雄醫學大學 === 醫學檢驗生物技術學系碩士班 === 103 === Metals are ubiquitous in food, industry, and the environment. Metals, lipids, and proteins are important biological and physiological components in organisms. Abnormal distributions of these analytes are disease factors and markers. Mass spectrometry (MS) can detect these analytes because of its high sensitivity, but different instrumentations are used to analyze inorganic metals and organic biomolecules. Using one analytical platform will make inorganic and organic MS analysis more simple and convenient. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect metals in real samples and to image tissues. Chelating agents were used to bind and ionize metals in food, drug, and water samples. This new technique successfully uses one analytical platform to analyze inorganic and organic analytes, also enabling a holistic understanding of tissues for clinical diagnosis. As the analysis of intact metal solids has not been performed using ambient MS, a technique known as electrospray laser desorption ionization mass spectrometry (ELDI/MS) was used to characterize metal solids without pretreatment. Metals in solid aluminum, copper, nickel, and lead samples were detected, as well as those in coins.