The Effects of Combined Curcumin and UVB on A375 Cell Lines

• Main Authors: Yu-Ci Huang, 黃于綺
• Other Authors: Kee-Lung Chang
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/c4jn82

碩士 === 高雄醫學大學 === 醫學系生物化學科碩士班 === 103 === Phototherapy is to expose skin in ultraviolet light (UV) on a regular basis and under medical supervision. The UV, ranged from 10 to 400 nano-meter, are divided into 3 groups of UVA, UVB, and UVC; and UVB radiation is the most common form of phototherapy and worldwide used to treat skin diseases especially for keratinocyte. UVB can penetrate the skin and slow the growth of affected skin cells and that has been proved in many other skin conditions. Curcumin, a substance in turmeric, is a polyphenol and commonly used as coloring agent and food additive. Extensive research has indicated this kind of polyphenol can be used for prevention and treatment of cancer. Previous studies indicated that curcumin might be a promising photosensitizer to induce apoptosis on human keratinocyte cell line HaCaT and there was synergistic effect of UVB and curcumin on induction of apoptosis in HaCaT cells. Up to now, the effects of combined curcumin and UVB on skin cancer cell, such as malignant melanoma, is still unknown. The present study was designed to investigate the effects of the combination of curcumin and UVB on human malignant melanoma cell line A375 and to explore the underlying mechanism. Our data showed that, after 24 h treatment with UVB irradiation or curcumin, the cell viability of A375 cells was decreased. Meanwhile, curcumin could enhance the cytotoxicity of UVB treatment. After co-treatment with75 mJ/cm2 UVB and 10 μM curcumin for 24h, cell morphology was changed and an oligonucleosomal DNA ladder was observed. Besides, the distribution of Sub-G1 in cell cycle, expression of apoptotic protein, Bax, and activation of pro-caspase-3 and pro-caspase-9 were increased; while expression of anti-apoptotic protein, Bcl-2 was decreased. These results indicated this combination stimulated A375 cell undergoing apoptosis. Furthermore, we found the combination would make citrate accumulation and ATP levels decrease which was owing to the disturbance of glycolysis by increasing in expression of Glut 1(Glucose transporter 1), G6PD (Glucose-6-phosphate dehydrogenase), T1GAR (TP53-inducible glycolysis and apoptosis regulator) and PDK (Pyruvate dehydrogenase kinase) and reuction in expressions of Hexokinase and PDH-E1&;#61537;&;#61472;&;#61480;Pyruvate dehydrogenase). Accordingly, we proposed that the induction of apoptosis by the combination is due to the decreased ATP production which resulted from the disturbance of glucose metabolism. Results of this study suggest that the combined treatment of curcumin and UVB has the potential for skin cancer therapy.