Development of A Technology Platform for Cellulosic Ethanol production
碩士 === 逢甲大學 === 生醫資訊暨生醫工程碩士學程 === 103 === This study was aimed to enhance the tolerance of Escherichia coli for the toxicity of lignocellulosic hydrolysate. This goal was achieved by the approach of metabolic engineering and directed evolution. As a result, one genetically-modified E.coli strain was...
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| Format: | Others |
| Language: | zh-TW |
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2015
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| Online Access: | http://ndltd.ncl.edu.tw/handle/ud77g4 |
| Summary: | 碩士 === 逢甲大學 === 生醫資訊暨生醫工程碩士學程 === 103 === This study was aimed to enhance the tolerance of Escherichia coli for the toxicity of lignocellulosic hydrolysate. This goal was achieved by the approach of metabolic engineering and directed evolution. As a result, one genetically-modified E.coli strain was obtained and enabled to undergo ethanol fermentation on 70% lignocellulosic hydrolyzate. Subsequently, this strain was immobilized.The immobilized strain was able to undergo ethanol fermentation on 100% lignocellulosic hydrolysate. Taken together, our result shows the potential of this strain for cellulosic ethanol production.
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