Nanomaterial Modified ElectrospunPoly(vinyl alcohol) Nanofibrous Membranes for Disposable Glucose Biosenser Application

• Main Authors: Chen Ting Chang, 陳廷彰
• Other Authors: Tsai Kun Hsieh
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/64034775780886294765

碩士 === 逢甲大學 === 纖維與複合材料學系 === 103 === The poly(vinyl alcohol) (PVA) electrospun nanofibrous membranes have demonstrated promising enzyme immobilization capability compared to the casted membranes because of its porous structure and large specific surface area. In this paper, we describe the fabrication of a disposable glucose biosensor based on the formation of electrospun nanofibrous membrane on a screen-printed carbon electrode where by polymeric mediator, PVA, and glucose oxidase (GOX)are homogeneously dispersed. The biosensor was evaluated with respect to sensitivity, dynamic range, reproducibility and stability by using Chronoamperometry. The morphology of PVA/GOx nanofibrous membrane was examined by SEM and TEM. Besides, to further increase the enzyme immobilization efficiency, conductive nanoparticles such as nanogold (Au) and graphene nanoplates were introduced to modify the PVA/GOx electrospun nanofibrous membrane. The influence of gold nanoparticles and graphene nanoplatesonthe sensitivity and selectivity of the biosensor was discussed.