| Summary: | 碩士 === 朝陽科技大學 === 應用化學系 === 103 === In this study, astragalus and ginger extract was added to the medium for the lactic acid bacteria Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus sporogenes to observe their influence on cell growth. Changes in the respective viability were observed during refrigeration.
Astragalus extract was added to the medium, and results showed that for B. bifidum grew best at 1500ppm, the viability increased from 7.82 108 CFU / mL to 2.03 109 CFU / mL. When aged ginger extract was added to the culture, at 3% addition, the viability of B. bifidum increased from 2.86 x109 CFU / mL to 3.44 x1010 CFU / mL. The effect of aged ginger extract on the other two species was not significant. The optimum concentration of 1% of the dried ginger extract added to medium for B. bifidum, cell concentration increased from 3.59 x109 CFU / mL to 6.23 x109 CFU / mL. When raw ginger extract was used at 5%, the growth enhancement for L. acidophilus and B. bifidum were respectively, from 8.35 109 CFU / mL to 1.45 x1010 CFU / mL, and from 1.74 x109 CFU / mL to 1.26 1010 CFU / mL.
The extracts were added to the refrigerated liquid cultures to observe the viability variations during refrigeration. The viability of L. acidophilus in the control group decreased. With added extracts, all species studied were still growing during refrigerated storage for 9 days.
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