| Summary: | 碩士 === 朝陽科技大學 === 應用化學系 === 103 === Hedychium coronarium Koenig is a monocotyledonous plant which belongs to the family of Zingiberaceae. The rhizome of H. coronarium is commonly used as the raw material to extract aromatic herbal oil. Hedychium plants also contain high level of sesquiterpene. The labdane diterpenes extracted from Hedychium coronarium have been reported to be vastly used in researches for their biological activities. In this work, the determination of total phenolic content, reducing power assays, as well as DPPH radical scavenging activity assay from different parts of Hedychium coronarium such as leaves, roots, stems, and flowers were investigated. Experimental results showed that distilled water extracts presented the best outcome: The highest total phenolic content was 73.51.5 μg GAEs/mg extract, which was obtained from leaves extracts, the best reduction ability came from leaves extracts, up to 82.90.3 μg Vit.C/mg extract; and flower extracts showed the highest DPPH radical scavenging ability, which reached 67.350.01%. Furthermore, a semi-preparative HPLC was used to isolated the constituents from Hedychium coronarium leaves extracts. Identification of compound structure was achieved by taking fraction (H.C.04) from NMR, FT-IR, and MS spectrum. The major component with molecular weight of 720 was determined. Additionally, the reduction in blood glucose level was conducted by applying a PPAR kit, and results showed that no significant difference between fractions H.C.03 and H.C.04 of Hedychium coronarium extracts.
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