Evaluating In Vitro Cytotoxicity of PAMAM (G1 and G2) Dendrimers on Mouse Blastocysts

碩士 === 中原大學 === 生物科技研究所 === 103 === Biological properties of dendrimers are highly important because the increased interest in using them for biomedical applications. Cationic dendrimers, like PAMAM, are generally haemolytic and cytotoxic. Their toxicity is generation-dependent and increases with th...

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Main Authors: Utami-Christina Vania, 范妮雅
Other Authors: Wen-Hsiung Chan
Format: Others
Language:en_US
Published: 2015
Online Access:http://ndltd.ncl.edu.tw/handle/82664242868128646843
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spelling ndltd-TW-103CYCU51110052016-08-22T04:17:15Z http://ndltd.ncl.edu.tw/handle/82664242868128646843 Evaluating In Vitro Cytotoxicity of PAMAM (G1 and G2) Dendrimers on Mouse Blastocysts 第一代與第二代樹狀高分子對小鼠囊胚之細胞毒性分析 Utami-Christina Vania 范妮雅 碩士 中原大學 生物科技研究所 103 Biological properties of dendrimers are highly important because the increased interest in using them for biomedical applications. Cationic dendrimers, like PAMAM, are generally haemolytic and cytotoxic. Their toxicity is generation-dependent and increases with the number of surface groups, suggesting that low generation dendrimers may be promising agents for targeted drug delivery. Conjugation with poly (ethylene glycol) (PEG) chains is also considered as a method of reducing the toxicity and increasing the biocompatibility of dendrimers. In this study to ensure its safety in biomedical usage, the low generation of PAMAM (G2) and PAMAM-PEG (G2) dendrimer, also PAMAM (G1) and Phosphorus (G1) dendrimer are evaluated for their cytotoxicity on mouse blastocysts. Embryos of ICR mouse in blastocyst stage are collected and exposed to various concentrations of all kinds of dendrimer (50 µg/ml, 100 µg/ml, 200 µg/ml, 300 µg/ml, and 400 µg/ml) and evaluated via TUNEL assay, Hoechst staining, and blastocyst outgrowth experiment. Blastocysts that were treated with PAMAM-PEG (G2) dendrimer and phosphorus (G1) dendrimer showing less apoptotic lesions and more cell proliferation number compared with the PAMAM (G2) and PAMAM (G1) respectively. The PEGylated dendrimer also showed better outgrowth (≥80% grade III outgrowth) compared with the unmodified one Wen-Hsiung Chan 詹文雄 2015 學位論文 ; thesis 55 en_US
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language en_US
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description 碩士 === 中原大學 === 生物科技研究所 === 103 === Biological properties of dendrimers are highly important because the increased interest in using them for biomedical applications. Cationic dendrimers, like PAMAM, are generally haemolytic and cytotoxic. Their toxicity is generation-dependent and increases with the number of surface groups, suggesting that low generation dendrimers may be promising agents for targeted drug delivery. Conjugation with poly (ethylene glycol) (PEG) chains is also considered as a method of reducing the toxicity and increasing the biocompatibility of dendrimers. In this study to ensure its safety in biomedical usage, the low generation of PAMAM (G2) and PAMAM-PEG (G2) dendrimer, also PAMAM (G1) and Phosphorus (G1) dendrimer are evaluated for their cytotoxicity on mouse blastocysts. Embryos of ICR mouse in blastocyst stage are collected and exposed to various concentrations of all kinds of dendrimer (50 µg/ml, 100 µg/ml, 200 µg/ml, 300 µg/ml, and 400 µg/ml) and evaluated via TUNEL assay, Hoechst staining, and blastocyst outgrowth experiment. Blastocysts that were treated with PAMAM-PEG (G2) dendrimer and phosphorus (G1) dendrimer showing less apoptotic lesions and more cell proliferation number compared with the PAMAM (G2) and PAMAM (G1) respectively. The PEGylated dendrimer also showed better outgrowth (≥80% grade III outgrowth) compared with the unmodified one
author2 Wen-Hsiung Chan
author_facet Wen-Hsiung Chan
Utami-Christina Vania
范妮雅
author Utami-Christina Vania
范妮雅
spellingShingle Utami-Christina Vania
范妮雅
Evaluating In Vitro Cytotoxicity of PAMAM (G1 and G2) Dendrimers on Mouse Blastocysts
author_sort Utami-Christina Vania
title Evaluating In Vitro Cytotoxicity of PAMAM (G1 and G2) Dendrimers on Mouse Blastocysts
title_short Evaluating In Vitro Cytotoxicity of PAMAM (G1 and G2) Dendrimers on Mouse Blastocysts
title_full Evaluating In Vitro Cytotoxicity of PAMAM (G1 and G2) Dendrimers on Mouse Blastocysts
title_fullStr Evaluating In Vitro Cytotoxicity of PAMAM (G1 and G2) Dendrimers on Mouse Blastocysts
title_full_unstemmed Evaluating In Vitro Cytotoxicity of PAMAM (G1 and G2) Dendrimers on Mouse Blastocysts
title_sort evaluating in vitro cytotoxicity of pamam (g1 and g2) dendrimers on mouse blastocysts
publishDate 2015
url http://ndltd.ncl.edu.tw/handle/82664242868128646843
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