| Summary: | 碩士 === 中原大學 === 化學研究所 === 103 === As time progresses, the rise of health conscious becomes more important in now days. Eating more vegetables and fruits can provide vitamin C, dietary fiber, carotenoids, polyphenols and other antioxidants. They are good for our health and rich in papaya tree. Papaya is a tropical fruit and planted in Taiwan. Papaya pulp is edible part of papaya tree and has antioxidant capacity. However, the other parts of the papaya trees, such as leaves, stems, peels, seeds are inedible. These parts also have antioxidant capacity. Therefore, I want to give new life to these wastes in this study. All parts of papaya trees are oven-dried in 55 ℃ and extracted by stirring extraction and ultrasonic extraction method. Then, the extracts are made an analysis of antioxidant capacity and total substance of antioxidants.
In antioxidant capacity case, Papaya leaf is the best one in DPPH radical scavenging activity and ferric reducing antioxidant power assay. The second one is unripe papaya seed. In total substance of antioxidants case, papaya leaves content more total phenols, flavonoids, condensed tannins and hydrolysable tannins than the unripe papaya seeds.
Discuss the supercritical extraction method to get the maximum amount of antioxidants in papaya leaves by Taguchi experimental design analysis. In this study, the best condition is under 200 atmospheres, 60 ℃, extracting for 2 hours, and modifier is H2O 200 μL for total phenols, condensed tannins and hydrolysable tannins. Another best condition is under 200 atmospheres, 60 ℃, extracting for 2 hours, without modifier for the total flavonoids.
In all extracting case, stirring extraction is the best method to extract the phenols, flavonoids, condensed tannins and hydrolysable tannins.
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