Analysis of protein solution extracted from plant de-oiled cake and microfiltration process by experimental design methodology

• Main Authors: Bo-Lin Chen, 陳柏霖
• Other Authors: Tsair-Wang Chung
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/r85wtj

碩士 === 中原大學 === 化學工程研究所 === 103 === In recent years, global energy crisis has approach to the alert zone. With the of energy exhaustion, human demand for energy is a considerable impact. Excessive use of fossil energy. Not only pollute the environment, but also result in decreasing the available energy. Biomass energy is a use of biomass crops or waste to convert into electricity and heat .Generally energy is mainly used to convert biomass is mainly organic matter produced by organisms. After the organic matter through the photosynthesis of plants to absorb solar energy, and then converted into chemical energy. To convert water, carbon dioxide and various soil minerals into various metabolites. Jatropha is an emerging biomass crops, it can be grown on marginal land, or areas of extreme temperature changes. Jatropha seed has high oil content. After a screw press, it can be separated from the crude oil and meal. Crude oil may pass through trans-esterification process to prepare biodiesel .The part of the meal contains a lot of protein (the crude protein content = 22.2%), it can be extracted by the extraction process to get large number of protein in meal. But Jatropha seed has toxic, so the absence through detoxification program, it can not let the protein as feed. In the present study, we will start with a single variable method for de-oiled cake Jatropha experiment. In order to achieve to make the oil content to a minimum, and consume less energy purposes. From experimental data, when temperature control at25 ℃, the number of replacement of hexane is one time ,and extraction time is 15 minutes, the oil content can be reduced to 0.581 % , After extraction program would use de-oiled cake proteins extracted into base solution , and use RSM to choose the better extraction parameter . From the regression of the experimental data know , when the extraction temperature was controlled at 30 ℃, the concentration of sodium hydroxide solution as 0.1M, solid-liquid ratio of 1:12, there will be a higher yield. In microfiltration process to remove slag, it also use RSM to choose the better parameter. When the feed pressure control in 2 kg/ (cm ^ 2), penetration of the tubular membrane pores size is 10μm. Protein will be relatively high transmission, and relatively low turbidity of protein solution. Then we would use the better parameters to increased membrane tube experiments to go long time operation, will add two tube membrane after 10μm tubular membrane, respectively for PP tubular membranes, and pore size are 0.5μm and 0.05μm . From the experimental data, we have to change the front three tubular membranes to avoid minimizing flux about 55 minutes. This dead-end filtration tubular membrane device compares to cross flow filtration device, it’s device is relatively simple, and can significantly reduce the production time, Because of its operating space compared to the cross-flow filtration device is more small ,and in the same processing volume . Cross-flow filtration system is required to maintain a very high flow rate, in order to gain considerable flux. The energy consumption will be relatively large, if under the same conditions, the device can significantly shorten the time preceding removal slag process.