| Summary: | 碩士 === 正修科技大學 === 化妝品與時尚彩妝研究所 === 103 === In this research, we use leaves of dried Encyclia cordigera as major materials and water as the solvent to proceed extractions for continuously three hours in three different temperature conditions at 30℃, 50℃ and 90℃, each with ratio of 1g:20g, to obtain the rough extracts after the last step, decompression and concentration. We then evaluate the antioxidative activity of the Encyclia Cordigera extracts in different temperatures from the test of DPPH free radical determination, Prussian blue restore capabilities, Total phenolic contents , Total flavonoid contents, Total carbohydrate and reducing sugar contents.
The result indicates that Encyclia cordigera extracts have antioxidative capacity under the same circumstances in every test above. In the test of DPPH free radical determination, Encyclia cordigera extracts are in the best condition at 90℃, IC50 with 40.28 ± 0.61 μg/mL, 50℃, IC50 with 67.49 ± 1.38 μg/mL comes the second, and 30℃with 106.77 ± 2.01 μg/mL is of the third. In the test of Prussian blue restore capabilities , Encyclia cordigera extracts are in the best condition at 90℃, EC50 with 109.68 ± 2.39 μg/mL, while there is no significant difference between the temperature at 50℃ water crude extract content of EC50 with 191.10 ± 2.73 μg/mL and at 30℃ water crude extract content of 187.50 ± 2.27 μg/mL is of the third. In the test of Total phenolic contents, Encyclia cordigera extracts are in the best condition at 90℃, 90℃ water crude extract content of 154.77 ± 1.14 mg of GAE/g, 50℃water crude extract content of 104.29 ± 0.65 mg of GAE/g comes the second, and 30℃water crude extract content of 98.66 ± 1.28 mg of GAE/g is the third. In the test of Total flavonoid contents , Encyclia cordigera extracts are in the best condition at 90℃,90℃ water crude extract content of 66.65 ± 0.67 mg of DC/g, 50℃ water crude extract content of with 36.20 ± 0.49 mg of DC/g comes the second, and 30℃water crude extract content of 98.66 ± 1.28 mg of DC/g is the third. In the test of Total carbohydrate contents, Encyclia cordigera extracts are in the best condition at 90℃,90℃ water crude extract content of 327.96 ± 1.30 mg of Glc/g, 50℃ water crude extract content of 257.17 ± 1.23 mg of Glc/g comes the second, and 30℃water crude extract content of 198.51 ± 1.51 mg of Glc /g is the third. In the test of Total reducing sugar contents , Encyclia cordigera extracts are in the best condition at 90℃,90℃ water crude extract content of 258.69 ± 0.59 mg of Glc/g,50℃ water crude extract content of 173.77 ± 0.43 mg of Glc/g comes the second, and at 30℃ water crude extract content of 163.69 ± 0.35 mg of Glc/g is the third. All of the above, it suggests that the antioxidative activity of Encyclia cordigera extracts are in the best condition at 90℃in each test. It shows that emulsion within Encyclia cordigera extracts does not cause skin irritation in the experiment to test the effectiveness. The statistics indicates that the emulsion within Encyclia cordigera extracts has moisturizing effect on the skin, in a week after the skin has obvious improvement effect of electric capacitance , and electric capacitance increase rate is about 22.79%, using four weeks after its electric capacitance increase rate of scale decreases to around 1.12%.While the elasticity of the skin also increases over time, after the first week of the elasticity of the skin increase about 6.25%, significantly higher than that of control group 3.37%. In the aspect of skin whitening, the skin melanin content before use average by 8.4 to 6 the melanin index reduced along with the increasing weeks of usage as well. Overall, emulsion within Encyclia cordigera extracts would be effective in skin moisture, elasticity, and skin whitening if applied to cosmetic formulations. Thus, how to add the extracts into the developing cosmetics in the future would worth further discussion and study.
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