I. Mechanisms of the Beneficial Effects of Ocimum gratissimum aqueous extract on rats with CCl4-induced acute liver injuryII. Effects of Human Parvovirus B19 And Bocavirus VP1 Unique Region On Tight Junction Of Human Airway Epithelial A549 Cells

• Main Authors: Chun-Ching Chiu, 邱駿清
• Other Authors: 徐再靜
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/04185308548567307464

博士 === 中山醫學大學 === 微生物免疫研究所 === 103 === Ocimum gratissimum (OG) is known as a food spice and traditional herb, which has been recommended for the treatment of various diseases. To investigate the hepatoprotective effect of OG aqueous extract (OGAE), male Wistar rats challenged by carbon tetrachloride (CCl4) were used as the animal model of hepatic injury. Our data imply that OGAE can effi-ciently inhibit CCl4-induced liver injuries in rats and may therefore be a potential food or herb for preventing liver injuries. As is widely recognized, human parvovirus B19 (B19) and human bocavirus (HBoV) are important human pathogens. Obviously, both VP1 unique region (VP1u) of B19 and HBoV exhibit the secreted phospholi-pase A2 (sPLA2)-like enzymatic activity and are recognized to participate in the pathogenesis of lower respiratory tract illnesses. However exactly how, both VP1u from B19 and HBoV affect tight junction has seldom been addressed. Therefore, this study investigates how B19-VP1u and HBoV-VP1u may affect the tight junction of the airway epithelial A549 cells by examining phospholipase A2 activity and transepithelial electrical resistance (TEER) as well as performing immunoblotting analyses. The results suggest that HBoV-VP1u rather than B19 VP1u likely plays more important roles in the disruption of tight junction in the airway tract. Meanwhile, this discrepancy appears not to be associated with the se-creted phospholipase A2 (sPLA2)-like enzymatic activity.