Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 103 === Raspberry, Rubus idaeus L., is a plant belonging to the Rosaceae family, genus Rubus. The purpose of this article is to evaluate anti-tumor effect of Rubus idaeus L. fruit. Rubus idaeus L. fruit was extracted by 50% ethanol(50% ethanol and 50% water) and anal...

Full description

Bibliographic Details
Main Authors: Min-hsiung Wang, 王敏雄
Other Authors: 謝易修
Format: Others
Language:zh-TW
Published: 2015
Online Access:http://ndltd.ncl.edu.tw/handle/93955712188163577895
id ndltd-TW-103CSMU5107024
record_format oai_dc
spelling ndltd-TW-103CSMU51070242016-02-24T04:18:36Z http://ndltd.ncl.edu.tw/handle/93955712188163577895 Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo 覆盆子酒精萃取物抑制人類肺癌細胞之MMP及u-PA表現及降低腫瘤之生長能力 Min-hsiung Wang 王敏雄 碩士 中山醫學大學 生化暨生物科技研究所 103 Raspberry, Rubus idaeus L., is a plant belonging to the Rosaceae family, genus Rubus. The purpose of this article is to evaluate anti-tumor effect of Rubus idaeus L. fruit. Rubus idaeus L. fruit was extracted by 50% ethanol(50% ethanol and 50% water) and analyzed by HPLC. The major compositions of Rubus idaeus extract(RIE) are gallic acid, epigallocatechin (EGC), catechin, p-coumaric acid. RIE has no cytotoxicity to human lung cancer cell A549 and H1299 under MTT assay. On Western blot analysis, RIE group has downregulation of MMP-2 /u-PA secretion and upregulation of TIMP-1/PAI-1 compared to control group. Furthermore, we used Boyden chamber to measure its cell invasion. Invasion ability in RIE group reduced both in A549 and H1299 cell line. The reduced invasion ability is positively correlation to concentration of RIE. In view of above mentioned study, RIE may inhibit tumor invasion. In mice animal study, less tumor weight was noted 40 days later. Immunohistochemistry revealed less Ki-67 performance in RIE group, indicating less cell proliferation .E-cadherin is a transmembrane glycoprotein, involved in cell-cell adhesion and epithelial-mesenchymal transition (EMT). Vimentin is highly expressed in mesenchymal cells and is positively correlated with increased metastasis. Cancer metastasis is the leading cause of death in all cancer patients. Immunohistochemistry also showed upregulation of E-cadherin and downregulation of vimentin in RIE group compared to control group. In conclusion, RIE has potential benefit in lung cancer treatment owing to reducing tumor proliferation and metastasis. Furthermore, it needs further detail study for involving signaling pathway. 謝易修 2015 學位論文 ; thesis 66 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 103 === Raspberry, Rubus idaeus L., is a plant belonging to the Rosaceae family, genus Rubus. The purpose of this article is to evaluate anti-tumor effect of Rubus idaeus L. fruit. Rubus idaeus L. fruit was extracted by 50% ethanol(50% ethanol and 50% water) and analyzed by HPLC. The major compositions of Rubus idaeus extract(RIE) are gallic acid, epigallocatechin (EGC), catechin, p-coumaric acid. RIE has no cytotoxicity to human lung cancer cell A549 and H1299 under MTT assay. On Western blot analysis, RIE group has downregulation of MMP-2 /u-PA secretion and upregulation of TIMP-1/PAI-1 compared to control group. Furthermore, we used Boyden chamber to measure its cell invasion. Invasion ability in RIE group reduced both in A549 and H1299 cell line. The reduced invasion ability is positively correlation to concentration of RIE. In view of above mentioned study, RIE may inhibit tumor invasion. In mice animal study, less tumor weight was noted 40 days later. Immunohistochemistry revealed less Ki-67 performance in RIE group, indicating less cell proliferation .E-cadherin is a transmembrane glycoprotein, involved in cell-cell adhesion and epithelial-mesenchymal transition (EMT). Vimentin is highly expressed in mesenchymal cells and is positively correlated with increased metastasis. Cancer metastasis is the leading cause of death in all cancer patients. Immunohistochemistry also showed upregulation of E-cadherin and downregulation of vimentin in RIE group compared to control group. In conclusion, RIE has potential benefit in lung cancer treatment owing to reducing tumor proliferation and metastasis. Furthermore, it needs further detail study for involving signaling pathway.
author2 謝易修
author_facet 謝易修
Min-hsiung Wang
王敏雄
author Min-hsiung Wang
王敏雄
spellingShingle Min-hsiung Wang
王敏雄
Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo
author_sort Min-hsiung Wang
title Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo
title_short Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo
title_full Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo
title_fullStr Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo
title_full_unstemmed Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo
title_sort rubus idaeus l. ethanolic extract inhibits mmp and u-pa of human lung cancer a549 cells and reduces tumor growth in vivo
publishDate 2015
url http://ndltd.ncl.edu.tw/handle/93955712188163577895
work_keys_str_mv AT minhsiungwang rubusidaeuslethanolicextractinhibitsmmpandupaofhumanlungcancera549cellsandreducestumorgrowthinvivo
AT wángmǐnxióng rubusidaeuslethanolicextractinhibitsmmpandupaofhumanlungcancera549cellsandreducestumorgrowthinvivo
AT minhsiungwang fùpénzijiǔjīngcuìqǔwùyìzhìrénlèifèiáixìbāozhīmmpjíupabiǎoxiànjíjiàngdīzhǒngliúzhīshēngzhǎngnénglì
AT wángmǐnxióng fùpénzijiǔjīngcuìqǔwùyìzhìrénlèifèiáixìbāozhīmmpjíupabiǎoxiànjíjiàngdīzhǒngliúzhīshēngzhǎngnénglì
_version_ 1718195970113536000