Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo
碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 103 === Raspberry, Rubus idaeus L., is a plant belonging to the Rosaceae family, genus Rubus. The purpose of this article is to evaluate anti-tumor effect of Rubus idaeus L. fruit. Rubus idaeus L. fruit was extracted by 50% ethanol(50% ethanol and 50% water) and anal...
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ndltd-TW-103CSMU51070242016-02-24T04:18:36Z http://ndltd.ncl.edu.tw/handle/93955712188163577895 Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo 覆盆子酒精萃取物抑制人類肺癌細胞之MMP及u-PA表現及降低腫瘤之生長能力 Min-hsiung Wang 王敏雄 碩士 中山醫學大學 生化暨生物科技研究所 103 Raspberry, Rubus idaeus L., is a plant belonging to the Rosaceae family, genus Rubus. The purpose of this article is to evaluate anti-tumor effect of Rubus idaeus L. fruit. Rubus idaeus L. fruit was extracted by 50% ethanol(50% ethanol and 50% water) and analyzed by HPLC. The major compositions of Rubus idaeus extract(RIE) are gallic acid, epigallocatechin (EGC), catechin, p-coumaric acid. RIE has no cytotoxicity to human lung cancer cell A549 and H1299 under MTT assay. On Western blot analysis, RIE group has downregulation of MMP-2 /u-PA secretion and upregulation of TIMP-1/PAI-1 compared to control group. Furthermore, we used Boyden chamber to measure its cell invasion. Invasion ability in RIE group reduced both in A549 and H1299 cell line. The reduced invasion ability is positively correlation to concentration of RIE. In view of above mentioned study, RIE may inhibit tumor invasion. In mice animal study, less tumor weight was noted 40 days later. Immunohistochemistry revealed less Ki-67 performance in RIE group, indicating less cell proliferation .E-cadherin is a transmembrane glycoprotein, involved in cell-cell adhesion and epithelial-mesenchymal transition (EMT). Vimentin is highly expressed in mesenchymal cells and is positively correlated with increased metastasis. Cancer metastasis is the leading cause of death in all cancer patients. Immunohistochemistry also showed upregulation of E-cadherin and downregulation of vimentin in RIE group compared to control group. In conclusion, RIE has potential benefit in lung cancer treatment owing to reducing tumor proliferation and metastasis. Furthermore, it needs further detail study for involving signaling pathway. 謝易修 2015 學位論文 ; thesis 66 zh-TW |
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碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 103 === Raspberry, Rubus idaeus L., is a plant belonging to the Rosaceae family, genus Rubus. The purpose of this article is to evaluate anti-tumor effect of Rubus idaeus L. fruit. Rubus idaeus L. fruit was extracted by 50% ethanol(50% ethanol and 50% water) and analyzed by HPLC. The major compositions of Rubus idaeus extract(RIE) are gallic acid, epigallocatechin (EGC), catechin, p-coumaric acid. RIE has no cytotoxicity to human lung cancer cell A549 and H1299 under MTT assay. On Western blot analysis, RIE group has downregulation of MMP-2 /u-PA secretion and upregulation of TIMP-1/PAI-1 compared to control group. Furthermore, we used Boyden chamber to measure its cell invasion. Invasion ability in RIE group reduced both in A549 and H1299 cell line. The reduced invasion ability is positively correlation to concentration of RIE. In view of above mentioned study, RIE may inhibit tumor invasion. In mice animal study, less tumor weight was noted 40 days later. Immunohistochemistry revealed less Ki-67 performance in RIE group, indicating less cell proliferation .E-cadherin is a transmembrane glycoprotein, involved in cell-cell adhesion and epithelial-mesenchymal transition (EMT). Vimentin is highly expressed in mesenchymal cells and is positively correlated with increased metastasis. Cancer metastasis is the leading cause of death in all cancer patients. Immunohistochemistry also showed upregulation of E-cadherin and downregulation of vimentin in RIE group compared to control group. In conclusion, RIE has potential benefit in lung cancer treatment owing to reducing tumor proliferation and metastasis. Furthermore, it needs further detail study for involving signaling pathway.
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author2 |
謝易修 |
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謝易修 Min-hsiung Wang 王敏雄 |
author |
Min-hsiung Wang 王敏雄 |
spellingShingle |
Min-hsiung Wang 王敏雄 Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo |
author_sort |
Min-hsiung Wang |
title |
Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo |
title_short |
Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo |
title_full |
Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo |
title_fullStr |
Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo |
title_full_unstemmed |
Rubus idaeus L. ethanolic extract inhibits MMP and u-PA of Human Lung Cancer A549 Cells and Reduces Tumor Growth in Vivo |
title_sort |
rubus idaeus l. ethanolic extract inhibits mmp and u-pa of human lung cancer a549 cells and reduces tumor growth in vivo |
publishDate |
2015 |
url |
http://ndltd.ncl.edu.tw/handle/93955712188163577895 |
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