| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 103 === Malignant glioma is one of the most lethal types of tumor in human. It has been shown that the highly metastasis and invasion result in death of the brain cancer patients. Thus, the find to candidate target genes may be necessary to understand the molecular mechanism of malignant transformation of human glioma tumors. PTX3 called tumor necrosis factor-inducible protein 14 gene (TSG-14) were mapped to chromosomes 24q28.3. PTX3 regulate cell growth, differentiation, invasion and apoptosis have been reported. However, its involvement in human glioma cancer progression remains unclear.To study the role of PTX3 in human malignant brain tumors, immunohistochemical staining from human brain cancer tissue array to found that PTX3 protein expression levels were both significantly upregulated in brain tumor tissues compared with normal brain tissues, and was positively correlated with differentiation (P<0.01) and tumor grade (P<0.01). We found that PTX3 protein expression level was significantly upregulated in GBM8401 cells than other glioma cells (U251, M059J, Hs683, U-87MG). Inhibition of endogenous PTX3 protein expression by using of RNAi assay, and we found that knockdown of PTX3 in GBM8401 cells decreased cell viability and induced cell cycle arrest in G0/G1 phase. Knockdown of PTX3 expression significantly inhibited cell migration and invasion, and inhibited the MMP-1 and MMP-2 expression in human GBM8401 cells. In addition, inhibiting of PTX3 were increased the phosphorylation of p38MAPK in GBM8401 cells.The result will be providing the PTX3 may serve as a tumor marker for poor prognosis for glioma tumor.
|
|---|
