Gallic acid inhibits the metastasis of colon cancer cells in mice

• Main Authors: Yu-Hong Chen, 陳昱宏
• Other Authors: 王朝鐘
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/83634712995523223178

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 103 === The colon cancer is cancer mortality rate death third, compare with other cancers as lung cancer, the large intestine and the colon cancer sieves examines is may prevent. If early discover colon cancer , the cure rate may reach as high as 90%.therefore how to cure the colon cancer and the discussion colon cancer cancer-causing possible, is clinical important research direction. The treatment of colon cancer operation excision as principle, chemistry treatment for assist, but cancer later period the survival rate low and after operation the cell easy to transfer, Merge traditional chemistry medicine treatment has serious side effect etc., make patient''s cure rate is low and relapse high. Therefore, the development a new medicine assists the treatment, the postponement or the suppression cancer development is essential. The natural anti-cancer research is the recent tidal current, gallic acid (GA) is the natural anti-oxidation ability phenolic compounds, can find in galla chinensis, lacquer tree, hamamelis , tea, ficus elastica discover...etc, be confirmed the toxicity responds poisonous to the cancer cell, but will not hurt the healthy cell. We conducted MTT assay, using the DLD-1 cancer of the intestines cell line to treated with different doses of GA, 3-OMGA, the 4-OMGA, observations DLD-1 cell viability, discovered three medicines do not have the obvious cell toxicity, then carries on the DLD-1 cell wound healing assay, we found that the long time, GA cells crawling ability weaker; Then in the metastasis experiment, observe from the transwell assay result, GA suppression cell transfer ability also is the effect best; In order to clarify its numerator mechanism, we analysis cytoskeletal proteins, adhesion molecules, and the specificity of inhibition of Src signal pathway to confirmed, GA via regulating CD44 signaling pathway effectively inhibited DLD-1 colon cancer cell metastasis effect. Finally also confirmed at the animal experimentation that, GA can inhibited cancer cell DLD-1 metastasis ability. According to this result GA has anti-colon cancer of the metastasis ability, future might apply in chemistry prevention becomes develops the new medicine.