The synergistic effects of promoting hard tissue regeneration of calcium silicate materials combining fibroblast growth factor-2

• Main Authors: Chao-Hsin Liu, 劉兆欣
• Other Authors: 高嘉澤
• Format: Others
• Language: en_US
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/67056765461776354807

博士 === 中山醫學大學 === 牙醫學系博士班 === 103 === Bone healing needs a complex interaction of growth factors that establishes an environment for efficient bone formation. We examine how calcium silicate (CS) and tricalcium phosphate (β-TCP) cements influence the behavior of human dental pulp cells (hDPCs) through fibroblast growth factor receptor (FGFR) and active MAPK pathways, in particular ERK. The hDPCs are cultured with β-TCP and CS, after which the cells’ viability and odontogenic differentiation markers are determined by using PrestoBlue® assay and western blot, respectively. The effect of small interfering RNA (siRNA) transfection targeting FGFR was also evaluated. The results showed CS promoted cell proliferation and enhances FGFR expression. It was also found that CS increases ERK and p38 activity in hDPCs. Furthermore, raises the expression and secretion of DSP, and DMP-1. Additionally, statistically significant differences (p < 0.05) have been found in the calcium deposition in si-FGFR transfection and ERK inhibitor between CS and β-TCP; these variations are indicated that ERK/MAPK signaling is involved in the silicon-induced odontogenic differentiation of hDPCs. In addition, Mineral trioxide aggregate (MTA) is a biocompatible material and has had several clinical applications in endodontic treatment since 1993. In this study, we examined the effects of MTA/fibroblast growth factor-2 (FGF-2) on material properties and in vitro hDPCs behavior. Setting time and DTS data were not found to be significant (p > 0.05) between MTA with and without FGF-2. Cell proliferation and differentiation increased significantly (p < 0.05) with FGF-2 mixed MTA. After siRNA transfection with FGFR, the proliferation and differentiation behavior of the hDPCs appreciably decreased when cultured on an MTA/FGF-2 composite. In contrast, no significant amounts (p > 0.05) of ALP and OC were secreted by hDPCs seeded on MTA. The current study shows that CS substrate plays a key role in odontoblastic differentiation of hDPCs through FGFR and modulate of ERK/MAPK activation and MTA/FGF-2 enhanced the higher expression of hDPCs proliferation and osteogenic differentiation as compared to pure MTA cement.