Anti-metastatic activity of Rheum palmatum L. extracts in human oral cancer cells

• Main Authors: Yang-Yu Chen, 陳姎愉
• Other Authors: Ciao-Wen LIn
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/80955195691202903870

碩士 === 中山醫學大學 === 口腔醫學研究所 === 103 === The incidence and mortality of oral cancer in Taiwan have been increased during the last decade, which could be mainly resulted from the difficulty in treatment related to metastasis. Rheum palmatum L, a traditional Chinese herbal medicine, has well effect of curing laxative; simulate the circulation of blood, stops bleeding, antitumor and anti-bacterial. Matrix metalloproteinases (MMPs), a family of zinc-dependent endopeptidase, are able to degrade the extracellular matrix which play critical roles in angiogenesis, cell growth, invasion and metastasis of cancer cells. Several studies demonstrated that the components of Rheum palmatum L induce apotosis in different human cancer cell lines as well as inhibit the ability of invasion and migration in human colorectal cancer cells. However, the effect of Rheum palmatum L on metastasis of oral cancer has yet to be fully clarified. Here, we investigated the anti-invasive activity of Rheum palmatum L on a human oral cancer cell line (SCC-9) in vitro and its underlying mechanisms. This study demonstrates that Rheum palmatum L extract. has no cytotoxicity at the range of 0~20 μg/mL in SCC-9 oral cancer cell line. According to western blot and zymography assays, an increase of Rheum palmatum L concentration effectively reduced the secretion and the protein expression of MMP-2. Furthermore, the expression of MMP-2 mRNA is substantially reduced by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR analysis . On the other hand, Rheum palmatum L extract. could inhibit the ability of invasion and migration in SCC-9 cell line via extracellular signal-regulated kinase (ERK) 1/2 signal pathway；moreover, could exert a considerable synergistic inhibition effect by treatment with the inhibitor of ERK U0126 and PD98059. Taken together, Rheum palmatum L extract. may provide a novel anti-metastasis agent in the treatment of oral cancer.