Application of Seawater Desalination Brine and Artificial brine in Microalgae Cultivation

• Main Authors: Kun-Hung Wu, 吳坤鴻
• Other Authors: Kuo-Jung Hsien
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/45476794767593439930

碩士 === 嘉南藥理大學 === 環境工程與科學系 === 103 === This study evaluates the feasibility of synthetic saline and real desalination brine applying in Dunaliella salina (D. salina) cultivation. D. salina cells are value to extract oil and carotenoid. The growth curves of D. salina in synthetic saline and real desalination brine tests are discussed. Oil and carotenoid production in cells are also determined. First, we try to find the suitable environmental parameters for D. salina. Different medium, light colors, light strength, and temperature are tested. The test mediums include Modified Johnsons medium (MJR), CNP medium (CNP), aeration (Air), and NP medium with aeration (Air+NP). The test light color includes red, white, and blue. Light strength ranges from 45 to 450 µmol photons m-2s-1. Cultivation temperature includes 25, 30, 35 ˚C. Cultivation salinity includes 35, 55, 60, 70, and 80 ‰. The results show that medium of AIR+NP, blue light, 70 ‰, and 30˚C is better for D. salina cultivation. And the light strength is the stronger the better between test periods of 45 to 450 µmol photons m-2s-1. Further, we use the real desalination brine for D. salina cultivation. The growth curve of D. salina in real desalination brine is similar to that in synthetic saline. It reveals that could live well in desalination brine. Besides the production of oil and carotenoid are also similar to that in synthetic saline tests. However, the salinity of the used desalination brine (55‰) is still too low to have better production of oil and carotenoid. The application of desalination brine in D. salina cultivation should consider to raise the salinity of the brine.