Development of a microfluidic system for the characterization of tumor cell invasion
碩士 === 長庚大學 === 醫療機電工程研究所 === 103 === In this study, a biochip was developed to mimic the basement membrane in order to study the invasion of cancer cells. During the invasion process, real-time and non-invasive impedimetric monitoring was concurrently performed to provide a quantitative measurement...
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2015
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| Online Access: | http://ndltd.ncl.edu.tw/handle/90856946570565294239 |
| Summary: | 碩士 === 長庚大學 === 醫療機電工程研究所 === 103 === In this study, a biochip was developed to mimic the basement membrane in order to study the invasion of cancer cells. During the invasion process, real-time and non-invasive impedimetric monitoring was concurrently performed to provide a quantitative measurement. The biochip consisted of a glass substrate and a fluidic layer. Microelectrodes were fabricated on the glass substrate for the impedance measurement. The fluidic layer was made of polydimethylsiloxane (PDMS) material by soft lithography. There were 2 culture chambers with a connecting channel in the biochip. The channel was filled with methylcellulose to mimic the basement membrane. Microelectrodes were located at the bottom of the channel for monitoring the invasion process. In this work, cancer cells (cell line: BM-1) were cultured on 1 chamber and culture medium was loaded to another chamber. After 6 hours for the cell adhesion, cells gradually migrated and invaded to the connecting channel filled with methylcellulose. Concurrently, impedimetric monitoring of the invasion process was performed to quantitatively analyze the cell invasion capability. This biochip has a high potential to be developed into an analyzing platform in the application of cancer research.
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