Novel Functionalized Self-assembled Nano Films

• Main Authors: Wen-Hao Chen, 陳雯灝
• Other Authors: Lai-Kwan Chau
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/7qbq4f

博士 === 國立中正大學 === 化學暨生物化學研究所 === 103 === Novel self-assembled bifunctional linker molecules have been successfully synthesized and experimentally demonstrated. The efficiency of multi-step surface modification of self-assembled nano films (SANFs) and the immobilization of metal nanoparticles on silica materials using three films has been greatly improved. The stability of three SANFs are also significantly improved. Most important, the processing of novel SANFs on the substrates is eco-friendly. Here, we characterized the SANFs on motified surfaces by contact angle, goniometer XPS and AFM. Comparison with the mercaptopropyl trimethoxysilane (MPTMS), the surface modification of mercaptopropyl silatrane (H-MPS) on the substrates is much faster and can be accomplished in a highly polar organic solvent (e.g., methanol and ethanol) due to the low moisture sensitiviey of silatrane. In addition, a functional group of R is used to protect the thiol group on MPS from further oxidation, and the resultant class of compounds is named R-MPS. The modification of R-MPS on the substrates can be achieved in an eco-friendly aqueous solution with a much higher efficiency than that of MPTMS. The second part of this research is to improve the structure stability and the antifouling capability of carboxyl betaine (CB) by adding a methyl group on the alpha side of its carboxyl group, and the produced compound is named Carboxyl-alpha methyl betaine (CAMB). X-ray photoelectron spectroscopy (XPS) and nuclear magnetic resonance (NMR) results show that CAMB presents higher chemical stability than CB in 1 M NaOH solution. What the mechamism of the Hofmann elimination on CB. The experimental serum (49 mg/mL) were then used for the non-specific adsorption tests. Both CB and CAMB have good antifouling capability against surrounding proteins. However, CB will gradually lose its antifouling ability due to the Hoffmen elimination reaction. In this study, CAMB is also applied to the immobilization of gold nanoparticles (AuNPs) on the fiber-optic particle plasmon resonance (FOPPR) sensor for bio-detection in diluted serum sample solutions. Experimental results successfully demonstrated that the CAMB-conjugated FOPPR sensor possesses a comparable sensitivity to the sensitivity of the conventional FOPPR sensor.