Exploring fermentation strategies for enhanced production of poly(4-hydroxybutyrate) by Bacillus drentensis CH38

• Main Authors: Chien-Han Chen, 陳建翰
• Other Authors: Yu-Hung Wei
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/5pqm66

碩士 === 元智大學 === 生物科技與工程研究所 === 102 === Polyhydroxyalkanoates (PHAs) are polyesters synthesized by various microorganisms such as Ralstonia eutropha, Alcaligenes latus, Aeromonas hydrophila, Pseudomonas putida and Bacillus spp. Because of their good biodegradability and biocompatibility, PHAs have attracted interest in their use as an alternative to petroleum-based plastics and as biomedical materials in varied applications, including fine chemicals, plastics, printing materials, bio-fuel, medical implants and drug delivery. The PHAs are energy storage materials that accumulate in bacteria under certain conditions such as insufficient nitrogen, phosphorus, sulfur and oxygen or excessive carbon. The aim of this study is to screen indigenous strains for PHA production. In this study, NileRed staining, Infrared (IR) and Gas Chromatography (GC) analysis suggested that the best strain for PHA synthesis is Bacillus drentensis CH38 , which obtains poly(4-hydroxybutyrate) (P(4HB)). The P(4HB) production of 5.2 g/L, the P(4HB) content of 41% and the cell dry weight (CDW) of 12.7 g/L were obtained, respectively, when the γ-butylactone contained medium was applied in this study. However, this study is needed to further evaluate the effects of γ-butylactone for P(4HB) production. The findings of this study provide a reference for further research in the use of P(4HB) for manufacturing biodegradable polymers.