Bioremediation of trinitrotoluene contaminated soil using indigenous microorganisms

• Main Authors: Bo-Hsin Lin, 林博歆
• Other Authors: Chih-Ching Chien
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/8zzsmq

碩士 === 元智大學 === 生物科技與工程研究所 === 102 === Soil and groundwater contaminated by munitions compounds has been a crucial issue in environmental protection. TNT (2, 4, 6-trinitrotoluene) is a secondary explosive widely used both for military and civil purposes all over the world. TNT contamination has been associated with adverse impacts on biological activities and human communities. Therefore, Control and remediation of TNT-pollution are critical issues in environmental protection. In this study, bioremediation approaches using indigenous microorganisms from TNT contaminated soil was evaluated by lab pilot-scale study for their efficiency and feasibility. Preliminary results showed that efficacy of degradation of TNT by indigenous microorganisms in the contaminated soil without any modification was insufficient. The addition of pure cultures of TNT-degrading microorganisms in the contaminated soil did not accelerate the degradation rate. However, the addition of extra nutrient supplement such as a carbon source (e.g. sucrose) could significantly increase the bioremediation efficiency. The results suggested that both TNT-degrading microorganisms and supplementation of sufficient nutrients might be a feasible bioremediation approach for TNT-contamination.