Preparation of dual growth factors released calcium phosphate coating onto Ti6Al4V by using electrochemical deposition

碩士 === 國立陽明大學 === 醫學工程研究所 === 102 === In this study, the calcium phosphate coating layer which had ability that sequential release of BMP-2 and TGF-β1 was deposited onto titanium substrate by using electrochemical co-precipitation method. It hypothesized that the incorporation of CHC could increase...

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Main Authors: Yen-Zhu Lin, 林晏竹
Other Authors: Tse-Ying Liu
Format: Others
Language:en_US
Published: 2014
Online Access:http://ndltd.ncl.edu.tw/handle/92675572579903782242
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spelling ndltd-TW-102YM0055300352015-10-13T23:50:23Z http://ndltd.ncl.edu.tw/handle/92675572579903782242 Preparation of dual growth factors released calcium phosphate coating onto Ti6Al4V by using electrochemical deposition 利用電化學沉積法於鈦合金基材上製備雙重生長因子釋放之磷酸鈣塗層 Yen-Zhu Lin 林晏竹 碩士 國立陽明大學 醫學工程研究所 102 In this study, the calcium phosphate coating layer which had ability that sequential release of BMP-2 and TGF-β1 was deposited onto titanium substrate by using electrochemical co-precipitation method. It hypothesized that the incorporation of CHC could increase the biocompatibility of calcium phosphate coating and accelerate its degradation rate. Different concentrations of CHC (0.02%, 0.06%, and 0.10%) were incorporated into calcium phosphate coatings. The chemical structure and crystalline of calcium phosphate/CHC coatings were investigated by FTIR and XRD. It was found that the crystallinity of calcium phosphate/CHC coating would decrease when the incorporation of CHC increased. Furthermore, the incorporation of 0.02% and 0.06% CHC into calcium phosphate coatings exhibited better cell proliferation and differentiation than hydroxyapatite coating in vitro. Lower crystallinity of calcium phosphate coating could release BMP-2 easier than higher crystallinity. PLA microspheres were used as carrier for second growth factor, TGF-β1. The release profile showed that in contrast with bare TGF-β1, TGF-β1 which was encapsulated by PLA microspheres revealed much slower release rate. The bioactive calcium phosphate coating embedding BMP-2 and PLA/TGF-β1 was successfully achieved long term sequential release of BMP-2 and TGF-β1. Additionally, the bioactive calcium phosphate coating embedding BMP-2 and PLA/TGF-β1 exhibited excellent cell viability and ALP activity when MC3T3-E1 cells were cultured on for 7-14 days. The technique of electrochemical co-precipitation and dual growth factors sequentially released calcium phosphate coating provide a promising potential for the clinical applications in bone implants and tissue engineering. Tse-Ying Liu Chung-Kwei Lin 劉澤英 林中魁 2014 學位論文 ; thesis 69 en_US
collection NDLTD
language en_US
format Others
sources NDLTD
description 碩士 === 國立陽明大學 === 醫學工程研究所 === 102 === In this study, the calcium phosphate coating layer which had ability that sequential release of BMP-2 and TGF-β1 was deposited onto titanium substrate by using electrochemical co-precipitation method. It hypothesized that the incorporation of CHC could increase the biocompatibility of calcium phosphate coating and accelerate its degradation rate. Different concentrations of CHC (0.02%, 0.06%, and 0.10%) were incorporated into calcium phosphate coatings. The chemical structure and crystalline of calcium phosphate/CHC coatings were investigated by FTIR and XRD. It was found that the crystallinity of calcium phosphate/CHC coating would decrease when the incorporation of CHC increased. Furthermore, the incorporation of 0.02% and 0.06% CHC into calcium phosphate coatings exhibited better cell proliferation and differentiation than hydroxyapatite coating in vitro. Lower crystallinity of calcium phosphate coating could release BMP-2 easier than higher crystallinity. PLA microspheres were used as carrier for second growth factor, TGF-β1. The release profile showed that in contrast with bare TGF-β1, TGF-β1 which was encapsulated by PLA microspheres revealed much slower release rate. The bioactive calcium phosphate coating embedding BMP-2 and PLA/TGF-β1 was successfully achieved long term sequential release of BMP-2 and TGF-β1. Additionally, the bioactive calcium phosphate coating embedding BMP-2 and PLA/TGF-β1 exhibited excellent cell viability and ALP activity when MC3T3-E1 cells were cultured on for 7-14 days. The technique of electrochemical co-precipitation and dual growth factors sequentially released calcium phosphate coating provide a promising potential for the clinical applications in bone implants and tissue engineering.
author2 Tse-Ying Liu
author_facet Tse-Ying Liu
Yen-Zhu Lin
林晏竹
author Yen-Zhu Lin
林晏竹
spellingShingle Yen-Zhu Lin
林晏竹
Preparation of dual growth factors released calcium phosphate coating onto Ti6Al4V by using electrochemical deposition
author_sort Yen-Zhu Lin
title Preparation of dual growth factors released calcium phosphate coating onto Ti6Al4V by using electrochemical deposition
title_short Preparation of dual growth factors released calcium phosphate coating onto Ti6Al4V by using electrochemical deposition
title_full Preparation of dual growth factors released calcium phosphate coating onto Ti6Al4V by using electrochemical deposition
title_fullStr Preparation of dual growth factors released calcium phosphate coating onto Ti6Al4V by using electrochemical deposition
title_full_unstemmed Preparation of dual growth factors released calcium phosphate coating onto Ti6Al4V by using electrochemical deposition
title_sort preparation of dual growth factors released calcium phosphate coating onto ti6al4v by using electrochemical deposition
publishDate 2014
url http://ndltd.ncl.edu.tw/handle/92675572579903782242
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