| Summary: | 碩士 === 國立陽明大學 === 醫學工程研究所 === 102 === In this study, the calcium phosphate coating layer which had ability that sequential release of BMP-2 and TGF-β1 was deposited onto titanium substrate by using electrochemical co-precipitation method. It hypothesized that the incorporation of CHC could increase the biocompatibility of calcium phosphate coating and accelerate its degradation rate. Different concentrations of CHC (0.02%, 0.06%, and 0.10%) were incorporated into calcium phosphate coatings. The chemical structure and crystalline of calcium phosphate/CHC coatings were investigated by FTIR and XRD. It was found that the crystallinity of calcium phosphate/CHC coating would decrease when the incorporation of CHC increased. Furthermore, the incorporation of 0.02% and 0.06% CHC into calcium phosphate coatings exhibited better cell proliferation and differentiation than hydroxyapatite coating in vitro. Lower crystallinity of calcium phosphate coating could release BMP-2 easier than higher crystallinity. PLA microspheres were used as carrier for second growth factor, TGF-β1. The release profile showed that in contrast with bare TGF-β1, TGF-β1 which was encapsulated by PLA microspheres revealed much slower release rate. The bioactive calcium phosphate coating embedding BMP-2 and PLA/TGF-β1 was successfully achieved long term sequential release of BMP-2 and TGF-β1. Additionally, the bioactive calcium phosphate coating embedding BMP-2 and PLA/TGF-β1 exhibited excellent cell viability and ALP activity when MC3T3-E1 cells were cultured on for 7-14 days. The technique of electrochemical co-precipitation and dual growth factors sequentially released calcium phosphate coating provide a promising potential for the clinical applications in bone implants and tissue engineering.
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