Regulation of Cell Morphology and Proliferation by Polypeptide Multilayer Films

• Main Authors: Shih-Ying Wang, 王詩瑩
• Other Authors: Chun-Min Lo
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/26j2tn

碩士 === 國立陽明大學 === 醫學工程研究所 === 102 === To date the manipulation of physical factors such as mechanical properties has been a trend in studying cell behaviors including cell adhesion, cell migration, cell proliferation and even cell differentiation. In this study, we cultured mouse induced pluripotent stem cells (iPSCs) on cross-linked polypeptide multilayer films, which were built up using cationic poly-l-lysine (PLL) and anionic poly-l-glutamic acid (PLGA) by means of layer-by-layer assembly, aiming to investigate how manipulating layer numbers and EDC concentrations influencing cell fates. Quartz crystal microbalance results certificated polypeptides indeed coated on the substrates step by steps during the depositing process. Atomic force microscopy showed the surface of the films were granular structure. Moreover, it also showed that both the number of film layers and the reaction of cross-linking caused the increasing of the surface roughness of the polypeptide multilayer films. Our results indicated that mouse iPSCs morphology and growth on polypeptide multilayer films could be regulated by the type of the outermost polypeptide layer, the number of layers in the film and the concentration of the cross-linkers.