The role of microRNA-338 in breast cancer carcinogenesis

• Main Authors: Lan-Shin Jiang, 姜嵐馨
• Other Authors: Tien-Shun Yeh
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/42430807219946084906

碩士 === 國立陽明大學 === 解剖學及細胞生物學研究所 === 102 === Carcinoma has been the leading cause of disease death in Taiwan, one of the carcinoma is breast cancer, it is a malignant tumor, and the mortality is high. The miRNA plays a critical role in cells, including development, differentiation, apoptosis, metabolism, proliferation, tumorigenesis and metastasis. Previous studies indicate that miRNA could be a biological marker or the predictor of prognosis and drug resistance in cancer. The study of miR-338 in cancer is rare, and there is no report in breast cancer. Therefore, we would like to investigate the role of miR-338 in breast cancer carcinogenesis. In this study, we found that overexpression of miR-338 would supprress cell growth, viability, migrated and invaded abilities. We also found that overexpression of miR-338 would not affect the cell cycle-associated proteins, but affected the EMT-associated proteins and decreased the YY1 oncogene expression, it showed that miR-338 may suppressed breast cancer carcinoma by targeting oncogene. Furthermore, miR-338 decreased the luciferase activity of YY1 3’UTR reporter plasmid, and also decreased YY1 expression both in mRNA and protein levels. Moreover, we used miR-338-3p or -5p inhibitors to study which one plays the critical role in breast cancer, we found that either inhibited miR-338-3p or miR-338-5p could increase migrated ability. Taken together, the results showed that miR-338 may act as tumor suppressor in breast cancer carcinogenesis.