Cell-QCM sensor to explore change in TEER value of cell growth process

• Main Authors: Yung-Yue Chang, 張擁樂
• Other Authors: Tsong-Rong Yan
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/54082109453396067500

碩士 === 大同大學 === 生物工程學系(所) === 102 === Quartz crystal microbalance (QCM) is a mass-sensitive and quickly response biosensor. A lab-made cell-QCM system, which can be used to culture animal cells and monitor the process of cells growth, division and death, was developed in our laboratory. In this study, the cell-QCM system was used to monitor the AC impedance profile during growth of the cultured cells. The data were compared to the data obtained from trans-epithelial electric resistance value (TEER value) with the MilliCellR chopstick electrodes. Three types of cell line, Caco-2, HepG2, and A375 were used in this study. The TEER value is used to evaluate the integrity of a monolayer of the culture cells. In the case of Caco-2, during 11th to16th culture-day was recognized as steady stationary state and in the case of HepG2 and A375, the stationary state was at 9th to 15th day, and 5th to 15 th day, respectively. Based on this data, 10μM of an anticancer drug, Mitoxantrone was added in the late stationary state of each culture cells, all of the TEER values were decreased rapidly. The same experiments were performed and the integrity of each monolayer was measured by the cell-QCM. One could find the stationary state was at 8.2 day to 13.2 day (Caco-2), 9.2 day to 11.3 day (HepG2) and 7 day to 10.5 day (A375), respectively. In each experiment, the anticancer drug, Motoxantrone was added; the integrity of each monolayer of cultured cells was decreased quickly. In a conclusion, both traditional TEER value and trans-membrane impedance by cell-QCM can monitor the growth curve of the cultured cells. The cell-QCM has more convenient, rapidly, and on-line monitor cell treated with drugs. It is suitable for the future drug screening and new drug development.