The study of inhibitory effects of human MCPIP3/ZC3H12C on the migration in colorectal cancer cells

• Main Authors: Ya-Ting chen, 陳雅婷
• Other Authors: Yan-Jhou Chen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/8ez9ur

碩士 === 臺北醫學大學 === 醫學科學研究所 === 102 === Monocyte chemotactic protein induced protein 3 (MCPIP3), also name as ZC3H12C (Zine finger CCCH domain-containing protein 12C), belongs to CCCH-zinc finger protein family member. MCPIP3 protein contains a highly conserved of the CCCH-zinc finger domain and Nedd4-BP1, YacP Nuclease (NYN) domain. Previous studies were shown that MCPIP3 inhibits the expression of pro-inflammatory genes, vascular cell adhesion molecule-1 (VCAM-1) and E-cadherin expression in endothelial cells, but the role and function of MCPIP3 in cancer cells are unknown. In human colorectal tissues. In previous experiments was found that most of cancer tissues are increased at the mRNA level of MCPIP3 compared to normal tissues. To understand the function of MCPIP3, our data investigated HA-MCPIP3/T-REx-293 cells which can be induced the MCPIP3 expression by doxycycline. Overexpression of MCPIP3 changed the epithelial-mesenchymal transition (EMT)-related marker genes expression correlated with reduced E-cadherin and increased Vimentin. To further investigate the functions of MCPIP3 in human colorectal cancer cells, overexpression of MCPIP3 by transduction of lentiviral in SW620 and HCT116 cell lines Overexpression of MCPIP3 significantly decreased cell migration and increased E-cadherin expression in both SW620 and HCT116 cells. These results suggest that MCPIP3 may play a negative role in cell proliferation and migration of human colorectal cancer cells.