| Summary: | 碩士 === 臺北醫學大學 === 醫學檢驗暨生物技術學系所 === 102 === To improve specific biomarkers for early diagnosis of lung adenocarcinoma is the main trend. The plasma levels of alpha-1-antichymotrypsin (AACT) were found could be a potential candidate in previous studies. In current study, immunoblot results demonstrated the correlation between high level of AACT and the plasma of lung cancer patients (16 of 18 cases, 88.9%); while a slight increase in AACT was found in the plasma of healthy volunteers (1 of 18 cases). The increase of the AACT level in late-stage patients was more obvious than in early-stage patients.
Besides the change of abundance, cancer-associated modifications of AACT could affect its physiological features in cancer cells. To survey unique posttranslational
modifications (PTMs) of AACT in the in the plasma of lung cancer patients, we used a Nano LC-MS/MS method. According to immunoprecipitation from the plasma of lung cancer patients and healthy volunteers, shows the different PTMs between two groups. Results indicate that AACT can be considered a potential marker for the diagnosis of lung adenocarcinoma. Not only influences of altered levels but also modifications of the function of AACT are worthy of through investigation.
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