| Summary: | 碩士 === 臺北醫學大學 === 醫學檢驗暨生物技術學系所 === 102 === Hepatitis C Virus (HCV) is an enveloped, single-stranded positive RNA virus that belonging to the Flaviviridae family; the human are main host. In 1989, Qui-Lim Choo and George Kuo et al., used a molecular cloning approach to identify the virion from the non-A, non-B hepatitis patient''s serum. According to published by CDC of Taiwan, it is estimated that 2% of the general Taiwan population are infected with HCV, and highest regional prevalence were reported in some area in southern Taiwan. Recently, the proportion of Taiwan''s population infected HCV are increased; Since 2004, a new form of chronic HCV infection named occult hepatitis C virus (OCI) was described by Castillo et al.. This infection is characterized by absence HCV RNA in serum, but with abnormality in liver function tests; however, about 70% of patients with OCI also have occurrence HCV-RNA in peripheral blood mononuclear cells (PBMCs) but it is not known if HCV is replicating in these cells. For this reason, the aim of this study design was to investigated the potential existence of occult hepatitis C virus infection (OCI) in blood donors with abnormal ALT levels and performed statistical analysis the prevalence of donors with HCV infection in the period between 2004 and 2013. Currently, all specimen using serum / plasma during laboratory experiment in Taipei Blood Center; in this study, we used a new specimen replaced serum by peripheral blood mononuclear cells (Peripheral blood mononuclear cells; PBMCs) which different from Taipei Blood Center''s specimen had detected the HCV RNA. Because HCV mainly invasive liver cells, the alanine aminotransferase (ALT) index will reveal an unstable pattern or high variability levels of clinical borderlines when human infected with HCV; therefore, it is a essential to issue that clinical significance of blood donors with abnormal ALT Index, whether physiologic or pathologic reasons. In order to avoid OCI cause hazard to the transfusion, enhanced safety and ensured quality of blood products; therefore, we offer this project as research topic of this thesis.
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