| Summary: | 碩士 === 國立臺北科技大學 === 生化與生醫工程研究所 === 102 === The application and manipulation of mesenchymal stem cells (MSCs) in tissue engineering is a novel treatment modality in a great variety of pathologies. They include a high quality repair with regeneration of the injured tissue but without fibrous tissue formation. Furthermore, MSCs have high proliferation potential, only a small number of cells are required with subsequent expansion ex-vivo prior implantation. Stem cells from human exfoliated deciduous teeth (SHEDs) had been isolated from the human exfoliated deciduous teeth. They exhibit multilineage differentiation capacity being capable to give rise to diverse cells like osteoblasts, chondrocytes, adipocytes, myocytes and possibly neural cells. There are many studies indicated that MSCs can differentiation into osteocytes. In this study, we hope that SHEDs can proliferation, differentiation and calcification into osteoblasts in 3D scaffold that is porous thermo- sensitive hydrogel including tricalcium phosphate or strontium phosphate with chitosan and β-glycerophosphate for cellular growth.
The used characteristic of fabricated scaffolds was measured by the meter of rheology and Scanning electron microscopy. After, SHEDs were seeded into the hydrogel, cell viability were measured by MTT assay for 1, 5 ,7 and 14 days respectively. The results appeared the cellular attachment well and no apparent cytotoxicity for fabricated hydrogels was found for SHEDs. After sustained culture to 21 days, there are obvious ALP activity and calcium deposits in containing strontium phosphate hydrogel was significantly higher than the other two groups. The expression of BMP-2 protein was observed at 7 days culture in all hydrogels.In addition, significantly increased expression of COL-1 (8.24 times) and RUNX2 (5.26 times) were in 14 days culture in strontium phosphate- containing hydrogel group .
To sum up, thermo- sensitive hydrogels can enhance the osteogenic. differentiation of human exfoliated deciduous teeth stem cells, and thus can be a powerful utilization in tissue engineering in the future.
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