The Study of D-lactate Fluorescence Biosensor Assay in Human Urine

• Main Authors: Han-Yin Yu, 余函穎
• Other Authors: 陳建銘
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/dy2bax

碩士 === 國立臺北科技大學 === 光電工程系研究所 === 102 === The purpose of this study is to develop a method using solid phase extraction to remove the impurities in urine to decrease fluorescent noise, then add the nicotinamide adenine dinucleotide (NAD+) and D-lactate dehydrogenase (D-LDH) in urine. D-LDH will catalyze D-lactate and NAD+ to pyruvate and NADH, and then using biosensor that use the wavelength of 340 nm UV-LED to excite NADH and release the fluorescence of 490 nm to detect the concentration of D-lactate in urine. This method has good linearity (R2=0.9979) and calibration range from 1 μM to 125 μM. Accuracy is between 93.22~110.54% and precision is between 2.04 - 20.83%. The linearity, accuracy and precision of this method are complied with rules of analysis. And compared to Two-dimensional HPLC system (2-D HPLC system) this method can reduce the determination time from 90 minutes to 1 minute each sample.