In Vitro Studies of Fibroblasts Embedded in Hydrogelic Fibers Made of RGD-modified Alginate

• Main Authors: Yen-Ying Li, 李彥瑩
• Other Authors: 林忻怡
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/zc86p7

碩士 === 國立臺北科技大學 === 生物科技研究所 === 102 === In tissue engineering, it is important that scaffolds require porosity, biocompatibility and degradability. Alginate is a natural material to fabricate scaffold. RGD peptide that can promote cell adhesion, cell growth and help wound healing is combined with some surface receptors of cell. For improve cell distribution, we mix the alginate and cells together. We modified alginate with RGD and embedded cells in hydrogel fibers made of RGD-alginate scaffold using rapid prototyping. Cell morphology and proliferation inside the RGD-alginate hydrogel were examined and compared to cells embedded in alginate. In this study, we knew the DNA quantification of the RGD-alginate is higher than alginate . Live/dead stain showed all cells are alive in two scaffolds. HE stain appeared the cell’s morphology no significant difference in two kinds of scaffolds. Results show that RGD-alginate scaffold is more stable for cell to growth.