Preparation and Characterization of Chitosan-Sodium Tripolyphosphate-Calcium-Vitamin D3 Nanoparticles

• Main Authors: Wei-Shan Hung, 洪偉珊
• Other Authors: Jin-Hwei Rwan
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/69516048290451428520

碩士 === 東海大學 === 食品科學系 === 102 === Chitosan(CS, original Mw 271 kDa, deacetylation ≧85%) and its hydrolateds (high Mw 200~250 kDa, medium Mw 100~150 kDa and low Mw≦ 50 kDa) were dissolved at a 2 mg/mL concentration and adjusted to pH 4.7,then individually mixed with calcium (Ca) solution and vitamin D3 (Vit.D3) solution at different concentration of 100~1000 mg/mL and 0.5~5.0 mg/mL, respectively. The mixed solution were added dropwise with sodium triployphosphate (TPP) solution (pH 9.0) at different CS/TPP mass ratio of 3:1, 4:1, 5:1 and 6:1 under stirring(150 rpm/15 min) to prepare CS-TPP-Ca-Vit.D3 nanoparticles. Such effective factors as Ca/Vit.D3 concentration ratio, CS molecular weight and CS/TPP mass ratio on the Ca and Vit.D3 encapsulation efficiency, particle size, zeta potential of the nanoparticles were investigated. Stability of the nanoparticles in the simulated gastric (pH 1.2,with pepsin) for 2 hrs and intestine (pH 7.4,with pancreatin continued for 6 hrs) system were determined. SEM of nanoparticles was also observed. As a result, firstly, on the Ca and Vit.D3 encapsulation efficiencies. The Ca encapsulation showed the lowest level at a Ca concentration of 700mg/mL, became increasing in both of using smaller or larger Ca concentrations and gave a range of 55.6~74.3%. The Vit.D3 encapsulation was 100% in all of Vit.D3 concentration. CSs of original Mw, high Mw, medium Mw and low Mw gave an encapsulation of 70.27,68.37,64.01 and 64.48%, respectively, but showed no influencing on the Vit.D3 encapsulation efficiency. On the CS-TPP-Ca- Vit.D3 nanoparticle size, it changed in a range of 360~580 nm as using Vit.D3/Ca concentration ratio from 0.5/100 to 4/800μg/ mg, but decreased to 495 nm with the concentration ratio 5/1000μg/ mg. The particle size gradually decreased when CS Mw became lower (1100~500 nm) and insignificantly affected by CS/TPP mass ratio (502~546.6 nm). On the zeta potential of the nanoparticles, it was not irregular affected by the Vit.D3/Ca concentration ratio (+18.5~+22.5 mV), became lower with the increasing CS/TPP mass ratio (+28.1~+24.1 mV), and increased with the increasing CS/TPP mass ratio (+24.1~+34.6 mV ). Moreover, CS-TPP-Ca- Vit.D3 nanoparticle that were prepared with low CS Mw, CS/TPP mass ratio of 6/1 and Vit.D3/Ca concentration ratio of 5/1000μg/ mg released almost 0.8% Ca and 1% Vit.D3 in the simulated gastric and intestine system.