Electrospun Biomedical Nanocomposite Fibers of Poly(Lactic Acid)/Hydroxyapatite

• Main Authors: Hsien-Chi Tsai, 蔡憲麒
• Other Authors: I.K. Yang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/58139976746914203905

碩士 === 東海大學 === 化學工程與材料工程學系 === 102 === The main purpose of this research is using a simple technique of electrospinning to prepare the PLA nano fibers and HA-PLA nano composite fibers, which as a scaffold of the three elements of tissue engineering for culturing osteoblast. The research divides into two parts: (A)The preparation of the fiber and composite fiber: To investigate the effect of solvent on electrospinning. DMF was added to chloroform in the ratio of D:C=1:4, the overall volatility of solvent was found decrease, and so that electrospinning can be carried out. Then the effects of various operating parameters were investigated. The images of SEM, showed that the diameter of spinning was mainly determined by the concentration of solution and the inner diameter of needle. Thus, approriate operating conditions were chosen in order to produce spun fibers with diameter approximating 400, 600 and 800 nanometers. By kepping the linear speed of the collecting drum at approximatedly 0.13, 5.17 and 10.50 m/s fiber membranes with orientational order parameter tensors approximately 0.06, 0.23 and 0.34 were obtained. To prepare composite fibers HSA was used as a surfactant to ensure the suspension of HA powder in chloroform and the suspension was then used to dissolve PLA until homogenous mixture was formed. The mixture was electrospun into nano composite fibers at the same conditions previously described. (B)The characterization of fibers: The content of HA powder in the composite fibers were calculated from the results of the TGA analysis. DSC analysis showed the degree of crystallinity increased with increasing fiber diameter. Crystallinity was also found increasing as the orientational order parameter tensors approached 0.5. The results of tensile analysis showed that when the orientational order parameter increased, Young’s modulus gained an increase of 200% to 300%. The Young’s modulus had a higher values when the diameter of fibers is smaller. In XRD analysis, the characteristic peaks of PLA and HA powder showed that the coexistence of both crystals with their inherent lattices. The final chapter, records the research about the effect of fibers orientation on the growth of osteoblast. The result showed that to an improved the survival or proliferated capacity of osteoblast accompanied with the increasing orientational order parameter of fibers, and the cell preferentially grew along the axis of fiber.