| Summary: | 碩士 === 慈濟大學 === 微生物學免疫學暨生物化學碩士班 === 102 === The spindle assembly checkpoint (SAC) is a surveillance mechanism that delays anaphase onset until all chromosomes are correctly attached in a bipolar manner to the mitotic spindle. Previous results showed that overexpression of LMO7 and its LIM domain peptide could cause a defect in the SAC, respectively. In this study, we aim to determine whether the defect in the SAC caused by overexpression of GFP-LIM is via its interaction with Afadin, YPEL1 or YWHAG. Afadin and YWHAG previously have been identified to interact with LMO7, and YPEL1, a spindle pole-associated protein, was identified as a LIM domain-interacting protein in our yeast two-hybrid screening. Fluorescence microscopic analysis revealed that GFP-LIM colocalized with Afadin, YWHAG and YPEL1 at the centrosomes in prometaphase and metaphase. Then, we found that down-expression of Afadin did not affect the SAC. Furthermore, we found that overexpression of YWHAG could not relieve the defect in the SAC caused by overexpression of GFP-LIM but overexpression of YPEL1 could exacerbate the defect in the SAC caused by overexpression of GFP-LIM. However, it remains to be determined whether the LIM-YPEL1 interaction is crucial for execution of the SAC
|
|---|
