Applying Experiment Design for the ParametersOptimization onGanoderma Lucidum Medium

• Main Authors: Liming Huang, 黃立銘
• Other Authors: 方正中
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/zra39u

碩士 === 南台科技大學 === 工業管理研究所 === 102 === Ganoderma has long been considered to have efficacy in the microbial fungi,in various circles is been long-term studies and discuss.Ganoderma has fairly extensive physiological activity and health functions, such as immune regulation, anti-tumor.Unlike traditional mushroom house that cultivation fruiting body needs to have a large areas and a lot of wood dust to cultivate.However, liquid Fermentation culture biomass more efficient and environmentally friendly.The use of modern biotechnology techniques to control environmental parameters, giving cultivate process quality more stable. The purpose of this study is to design experiments for research medium formulation to gaining the best elements of the proportion of medium formulation then get the maximum amount of biomass.The respective elements of the study was carbon (glucose), nitrogen (Yeast extract), microelement (MgSO4).For biomass maximize research, the most efficient way to find the least cost of production parameters to achieve the best yield of the largest, and allows stable product quality. In this study, three standard three-factor experimental design to carbon, nitrogen, trace elements as a factor. ANOVA analysis of experimental data, a significant factor, by regression analysis to identify important influence affecting biomass dry weight variables and forecast mode and optimized to get the best medium proportions, and finally confirmation test. After improving the results of this study show that the best of the best medium ratio is carbon 10%, nitrogen1%, microelement 0.01%, making the original biomass dry weight yield 3.54g / l increased by 66.7%, reaching 5.90g / l.