| Summary: | 碩士 === 實踐大學 === 食品營養與保健生技學系碩士班 === 102 === The prevalence of type 2 diabetes is increasing after the 20th century. Insulin resistance is powerful indicatior for type 2 diabetes development, and also a therapeutic target nowadays. Several studies indicated that the supplementation of lactic acid bacteria effectively improved hyperglycemia, high oxidant stress, insulin resistance, and type 2 diabetes mellitus. However, the mechanism of anti-insulin-resistance effect of lactic acid bacteria is poorly understood. In the present study, we evaluated the effect of Lactobacillus reuteri ADR-1 and the possible mechanisms in the improvenment of insulin resistance. The male Spraue-dawley rats were given low dose streptozotocin (15 mg/kg) and nicotinamide (45 mg/kg) by intraperitoneal injection, and fed with high-fat-diet 8 weeks to induce insulin resistnace. Thirty male Sprague-dawley rats with insulin resistance were equally divided into three groups and given either water (IR group),1.6 x 107 CFU/ml of Lactobacillus reuteri ADR-1 (0.1x ADR-1 group),1.6 x 108 CFU/ml of Lactobacillus reuteri ADR-1 (1x ADR-1 group), or 8 x 108 CFU/ml of Lactobacillus reuteri ADR-1 (5x ADR-1 group).The results showed that β-cell mass and the mRNA levels of ppar-α, ppar-γ, and Cyp7a1 were higher in the ADR-1 treated groups than in IR group. In contract, the ADR-1 treated groups had lower levels of FBG, OGTT, HOMA-IR, TC, TG, LDL, TBARS, pro-inflammation cytokines (TNF-α、IL-1β、IL-6), and liver weight, and fatty aid synthesis genes (SREBP-1, CHREBP,FAS) in ADR-1 treated groups. Moreover, the rats of 5x ADR-1 grouphad lower HbA1c and kidney weight than those of IR group had. The results provided evidences clarifying the effectivence of Lactobacillus reuteri ADR-1 can improve insulin resistance by mitigation of β-cell damage, enhancement of anti-oxidation acitivity modulation of inflammation and regulation of metabolic genes expression.
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