Effects of Different Cluster Power Clean on Biomarkers of Endothelial Damage and Fatigue in Collegiate Male Basketball Players

• Main Authors: Weng, Ming-Chia, 翁明嘉
• Other Authors: Chiang, Jasson
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/73918749349108492744

博士 === 中國文化大學 === 體育學系運動教練碩博士班 === 102 === Abstract Background: Bone marrow-derived, circulating endothelial progenitor cells (EPCs) contributed to the maintenance of endothelial function and organ perfusion by mechanisms ranging from endothelial repair to angiogenesis and vasculogenesis. Although exercise has been observed to modulate EPCs numbers in the circulating blood, yet no data were available so far on the amounts of circulating EPCs and circulating endothelial cells (CECs) responses to the power clean exercise. Purpose: The current study investigated the effects of traditional power clean (TPC) and interval cluster power clean (CPC) on the mobilization of EPCs and CECs subsets into the peripheral blood compartment and biomarkers of muscular damage and fatigue. Methods: Fifteen collegiate male basketball players (19.29 ± 1.30 years old) were recruited and randomly distributed and underwent two different configurations of power clean: Traditional power clean (TPC): Total volume = 8 sets x 5 repetitions x 80% of 1 RM (intensity) with intra-set rest: 240 seconds and inter-repetition rest interval: 0 second; Interval cluster power clean (CPC): Total volume = 8 sets x 5/1 repetitions x 80% of 1RM (intensity) with intra-set rest: 120 seconds and inter-repetition rest interval: 30 seconds. After signing the inform consent form for this study, the physical activity readiness questionnaire (PAR-Q) and 3 repetitions of one maximum muscular strength were accessed for the selected subjects prior to the study, and blood pressure and heart rate values were monitored during conducting each protocol. This research has been approved by Mackay Memorial Hospital Institutional Review Board (IRB) Approval of clinical trial. The means of blood drawing were divided into vein detained needle on forearm using 3 c.c. venous blood samples which were drawn in-time, and at 10, 30, 60 minutes, and 24 hours after completing condition protocol for testing EPCs and immunological biomarkers; meanwhile, fingertip puncture to test the capillary blood samples for lactate concentration were obtained immediately, and at 3, 5, 7, as well as 10 minutes after exercise. Blood samples for measurement of EPCs and CECs were analyzed by three-color flow cytometry and biomarkers of muscular damage and fatigue such as creatine-phospho-kinase (CPK), lactic dehydrogenase (LDH), aspartate glutamate oxaloacetate transaminase (GOT), C- reactive protein (CRP), serum urea nitrogen, (SUN), and creatinine (CRE) were measured by Fuji Dri-Chen Clinical Chemistry Analyzer. Repeated measures two-way analysis of variance (ANOVA), mixed design was used to exam the differences between two configurations as independent variables and physiological biomarkers as dependent variables. Results: 1) TPC elicited higher amounts of EPCs than CPC, but not shown significant difference (F=2.84, p>.05); meanwhile the highest value of both two exercises in EPCs appeared at 10 minutes after performing each power clean configuration. 2) CPC elicited higher amounts of CECs than TPC and shown significant difference (F=835.14, p <.05). Otherwise, the peak value of CECs of both two groups appeared at 30 minutes after exercise. 3) The values of heart rate, systolic and diastolic blood pressure were significantly affected by TPC and CPC protocols (p<.05). 4) The blood lactate did differ between TPC and CPC configuration (F=1179.29, p<.05) and the peak value of TPC appeared at 3 minutes (9.87±1.98 mmol/L) but CPC at immediately (6.53±0.62 mmol/L) after exercise. 5) In aspect of muscular damage biomarkers: The average CPK, LDH, and GOT values of CPC group were higher than TPC group without showing significant difference (p>.05). The highest value of CPK of TPC and CPC presented at 24 hours. LDH of TPC obtained the highest value at 60 minutes, but CPC at in-time after exercise. The highest value of GOT in both group appeared at in-time. 6) For fatigue-induced biomarkers: CRP was below the detected value (0.3 U/L) by the chemical analyzer, so this research cannot prove the effects of two protocols on CRP. The highest ratio of SUN/CRE of TPC was at 60 minutes but CPC appeared at before the exercise. 7) Both two groups also caused dehydration significantly. TPC decreased 1.70±0.42 kg (t=15.71, p<.05) and CPC decreased 1.21±0.44 kg. Conclusion: This study clarified the effects of TPC and CPC with intervening different rest interval between each set or per repetition on EPCs, CECs and biomarkers of muscular damage and fatigue. It elucidated that the interval cluster power clean was indeed a safer and physical activity-friendly exercise relevant to enhance the endothelial functions. Keywords: Explosive power exercise, endothelial progenitor cells, circulating endothelial cells