The Implementation of Nanochannel Electroporation (NEP) Chip on Cell in Vivo

• Main Authors: Yu-Chi Chang, 張聿騏
• Other Authors: I-En Lin
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/n9f7cg

碩士 === 國立虎尾科技大學 === 機械與機電工程研究所 === 102 === Electroporation is a method used in gene transfer, increased cell surface pores to expand by changing electric field. The exogenous substances (plastid DNA ... etc) transgenic into cells. This study physical transgenic electrical impulses membrane perforation method as discussed. Jurkat cells were performed on the NEP chip, the electric pulse perforated membrane, having a fluorescence-modified oligonucleotide synthesis section (Oligo nucleus) in the cell transfer, to achieve operation of the fluorescent marker. NEP wafer used in this study contains a microchannel, a total of 20 groups of nanochannel array structure, and in vivo cells were injected into both ends of each groove in the glass slide substrates. After the electrical pulse transgenic membrane perforation, in inverted fluorescence microscope observation. Experimental results show that the proportion of transgenic average 34.83%. Taguchi method with experimental design, analysis of the results is an important factor that voltage. From the results also found that the higher the voltage value is smaller transgenic ratio.