| Summary: | 碩士 === 國立虎尾科技大學 === 生物科技研究所 === 102 === Medicinal mushrooms have attracted much attention recently owing to their potent therapeutic activity, especially as chemotherapy and immunomodulatory agents. Antrodia cinnamomea is an endemic medicinal fungus that grows mainly inside the rotten trunk of Cinnamomum kanehirae in natural conditions in Taiwan. Due to the limited distribution of the host plant and the slow growth rate of the fungus, the mass production of the fungus through in vitro culture systems including on the artificial cultural camphor tree for pharmaceutical usage has been attempted. Asarum heterotropoides F. Schmidt var. mandshuricumon that was assigned to birthwort family Aristolochiaceae, have been reported to promote growth of A. cinnamomea, also named Taiwanofungus camphorates. Therefore, the purpose of this study is to determine the effect of A. heterotropoides (Ashe) on sporulation and mycelial growth of A. cinnamomea in vitro and to detect the shorten growth time and formation of fruiting body quality compared with A. cinnamomea in natural.
For sporulation and mycelial growth tests, two different strains of A. cinnamomea: AC1CK and AC-N were used and cultured with the extract from A. heterotropoides (Ashe). The malt extract agar (MEA) and a series of dilution of Ashe were made to culture for A. cinnamomea spores. Five fractions of Ashe, including Ashe E, Ashe W, Ashe C, Ashe CH, Ashe CM with different concentration 500 ppm, 1000 ppm and 2000 ppm were tested in this study. The results indicated that strain AC1CK and strain AC-N grew fastest in 2000 ppm among tested concentration as compared with other dosage. Ashe CH was the best among the partial fraction of Ashe to promote mycelial growth of A. cinnamomea at 2000 ppm. For mycelial growth tests, the results revealed that the Ashe CH obviously promoted the mycelial growth of A. cinnamomea to 99.28 -150.36 % in dose dependent manner with control. For pH tests, the maximum yield of 908 mg/100 ml, and 929 mg/100 ml, occurred at pH 5.0 in PDB with 2000 ppm Ashe CH of both strains AC1CK and AC-N.
Furthermore, pure compounds from Ashe CH, asarinin and sesamin were used at concentrate 500 ppm to detect the effect on spore germination. The results indicated that spore germination rate of A. cinnamomea could be promoted to 120% as compared with untreated control. Based on the above results, the reliable production of the medicinal fungus can promote by Ashe treatment under laboratory conditions.
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