Effects of plant growth regulators and chemical additives on in vitro morphogenesis from callus of Panax ginseng

• Main Authors: Jheng-jie Jiang, 江政杰
• Other Authors: Jen-Tsung Chen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/71839150152255503891

碩士 === 國立高雄大學 === 生命科學系碩士班 === 102 === Calli of Panax ginseng were used as explants to study the effects of ascorabic acid (AA), activated charcoal (AC) and polyvinylpyrrolidone (PVP) on proliferation rate and in vitro morphogenesis. All the explants were cultured on a modified MS (Murashige and Skoog, 1962) medium in darkness. After four weeks of culture, 100 mg/L AA gave the highest proliferation rate with an average of 9.14 folds on the callus line which was originally induced at 0.5 mg/L 2,4-D + 0.1 mg/L TDZ. Callus cultured on MS medium supplemented with 0.5 mg/L 2,4-D, 0.5 mg/L TDZ and 50 mg/L AA had highest proliferation rate with an average of 13.69 folds. Callus cultured on MS medium supplemented with 2 mg/L 2,4-D, 0.5 mg/L TDZ and 50 mg/L PVP had highest proliferation rate with an average of 7.1 folds. We selected the callus which induced by the concentration of 0.5 mg/L 2,4-D + 0.1 mg/L TDZ, 0.5 mg/L 2,4-D + 0.5 mg/L TDZ and 2 mg/L 2,4-D + 0.5 mg/L TDZ with different ratio of cytokinins and auxins medium for in vitro morphogenesis. Under the condition of the callus which induced by the concentration of 2 mg/L 2,4-D + 0.5 mg/L TDZ after 5 months were cultured on MS medium with 0.02 mg/L Kinetin + 3 mg/L NAA, the treatment could promote root formation and had of 6.34 roots in average. In addition, the callus treated with 0.5 mg/L 2,4-D + 0.1 mg/L TDZ or 0.5 mg/L 2,4-D + 0.5mg/L TDZ were cultured on MS medium with all different ratio of kinetin + IBA or Kinetin + 2,4-D did not possess the ability of root formation, but callus turned green. The result showed that root induction is depends on the culture time while not the high ration of NAA/kinetin.